264 



ORG \ NIC Ik ON COMPOUNDS IN TLAN IS. 



and the alkaline extract was acidified with acetic acid whereby 

 the crude ferro-nuclein was obtained : — 



This precipitate was however probably not yet pure. 



The other portion {b) was not previously deprived of its 

 starch content, but directly subjected to artificial pepsin diges- 

 tion, the -'insoluble residue treated with o. \% caustic potash, 

 acidified with acetic acid and the precipitate thus produced, was 

 washed with water, alcohol and ether. Nearly 7 grams of 

 impure ferro-nuclein resembling closely that of (a) was thus 

 obtained. 



Thus from 100 grams of dry sample I obtained nearly 10 

 grams of impure ferro-nuclein with about o.g% iron. 



(4). 0.2 grams of the crude ferro-nuclein (containing 0.5%" 

 iron and nitrogen) of Polygonum seeds 1 was treated with 

 100 CC. of 0.2^0 hydrochloric acid, and divided into two parts. 

 To one part, was added some pepsin and both were kept for 

 twenty hours at the temperature of 37 — 40 0 , whereby a small 

 portion of the iron was evidently splitt off by the action of the 

 acid from the original compound, as the ferro-cyanide test 

 clearly proved. It was further observed that the albumoses and 

 peptones formed by the action of pepsin contained iron in 

 organic combination. 



These experiments were repeated with several other samples 

 with essentially the same result. Since the haematogens of 

 Bunge and of Stoklasa do not undergo any change by artificial 

 digestion and as they do not so easily splitt off some iron by 

 the action of 0.2^ hydrochloric acid, my ferro-nuclein differs 

 evidently from the former, especially since it is not easily soluble 

 in dilute ammonia. 



The existence of apparently the same ferro-nuclein in the 

 seeds of Indigofera tinctoria, etiolated shoots of Polygonum 

 tinctorium, and in the full grown leaves of Indigofera and 

 Polygonum w r as shown by my further experiments. Similar 



Asli 



Fe 2 0 



Fe 



3 



4 9% 

 091% 



1 and 0.1 grams of that of indigofera seed. 



