342 



S. SAW AMUR A. 



used for experiments as in the former case. To test the 

 digestion of fat, olive oil was used. For this purpose a little 

 soda solution was added to the olive oil, and after the mixture 

 was well shaken it was dissolved in ether. The ethereal 

 solution was separated, washed with water, and the ether left 

 to spontaneous evaporation. The neutral olive oil thus obtained 

 was mixed with some alkaline enzyme-solution in a small glass 

 tube, a little azolithmin solution being added to indicate the 

 change of the reaction of the solution. After 8 days the results 

 observed were as follows : — 



Solutions. 



Material 

 used. 



Temperature. 



Results. 



0.4% HC1. 



fibrin. 



36 0 c 



not dissolved. 



Acid solution of 

 the enzymes. 









'• 



" 



room- 

 temperature. 



>* 



> f 



starch. 



>, 



not changed. 



0.3% solution of 

 sodium carbonate. 



fibrin. 



36° C. 



not dissolved. 



Alkaline solution of 

 the enzymes. 







dissolved. 







room- 

 temperature. 







starch. 



36° C. 



starch 

 disappeared. 



0 



fat. 



•• 



acid set free. 



Gelatine was liquefied on bringing it in contact with some 

 of the alkaline solution of the enzymes in the presence of some 

 thymol. Also freshly precipitated casein was easily dissolved 

 by it. 



