344 



S. SAWAMURA. 



From these results it may be concluded, that there exists 

 a difference between the enzyme production of the intestines and 

 that of the stomach, no diastase and lipase being present in 

 the former. 



Another experiment was made with the intestines of 

 Caligula japonica Moor. 6 grs. of the intestines were washed 

 in water, crushed in a mortar together with glass powder, and 

 digested with 4C\% alcohol. After seven days it was filtered, and 

 to the filtrate ether-alcohol (i part of ether and 3 parts of 

 alcohol) was added. The precipitate collected on a filter was 

 exposed to the air for a short time to evaporate the alcohol, and 

 then divided into two parts. One part was dissolved in 20 

 c.c. of o. \% HCI, and the other in 20 c.c. of o.^% solution 

 of sodium carbonate. The tests were made as in the former 

 experiments, and the mixture kept in a thermostat for six days. 

 The results observed were as follows: — 



Solutions. 



Materials 

 used. 



Results. 



0.1% HCI. 



coagulated 

 egg-albumcn. 



not corroded. 



Acid solution of 

 the enzymes. 



?» 



» 





fibrin. 



not dissolved. 



»» 



starch. 



not changed. 



°-3% solution of 

 sodium carbonate. 



coagulated 

 egg-albumen. 



not corroded. 



Alkaline solution of 

 Ihe enzymes. 





corroded and biuret 

 reaction produced. 





fibrin. 



dissolved and biuret 

 reaction produced. 





starch. 



not changed. 





fat. 



acid was not 

 set free. 



