ASPARAGIN IN THE METABOLISM OF SHOOTS. 353 



(a) Original, dried at the beginning of the experiment. 

 Number of shoots 250, average length 24 cm., dry weight = 

 5.4126 g. 



(/?) Control. Kept 45 hours in distilled water in darkness 

 Number of shoots 250, average length 24 cm., dry weight = 



5-1355 S- 



(c) Test shoots. Kept 45 hours in darkness, deprived of. 

 oxygen. Number of shoots 250, average length 24 cm., dry 

 weight =4.678 g. 



The analysis gave the following results : 





In 100 parts of dry matter 



For ico parts of total nitrogen 





Original. 



Control. 



Test shoots. 



Original. 



Control. 



Test shoots. 



Total nitrogen 



5.00 



4.90 



545 



100.0 



100 0 



100.0 



Albuminoid 



nitrogen ... 



2.46 



1.99 



2.27 



49.20 



40.61 



• 41-65 



Asparagin 



nitrogen ... 



1.40 



1.88 



1.52 



27.96 



38.12 



27.90 



Ammonia 



nitrogen ... 



0.20 



0.17 



0.22 



4.00 



3-55 



4.04 



Amido nitrogenf... 



0.94 



0.86 



1.44 



18.84 



17.72 



26.42 





Asparagin. 





In 100 parts of dry matter 



Every 100 shoots contain 





8.85 



0.1818 



In absence of air... 



7.16 



0.1336 



Original 



6.59 



0.1427 



1 The shoots were nearly dead, but the roots were still fresh. 



■f Amido-nitrogen means here nitrogen in the form of the primary amido-compounds 

 formed from protein. 



