AspArAGin In the metabolism of shoots. 355 

 Nitrogen in form of the primary amido-compounds. 





Iiarley. 



Soy bean. 





In 100 parts of 



In 100 parts of 



In 100 parts of 



In j 00 parts of 





dry matter 



total nitrogen 



dry matter 



total nitrogen 



With oxygen 



0.86 



17.72 



°-93 



9-5 1 



Without oxygen 



1.44 



26.42 



1.68 



iS-Si 



The following conclusion may be drawn from these results : 

 In etiolated shoots, the decomposition of protein continues 

 in the absence of oxygen as well as in its presence. This is in 

 accordance with what we know of the action of enzymes. The 

 shoots had remained ajaparently healthy for 45 — 52 hours when 

 deprived of oxygen. Only some shoots had lost turgor, but these 

 were quite free from any bacterial attack. The roots were 

 in contact with but little water and retained their normal 

 appearance. A very decisive difference in the production of 

 asparagin was however noticeable : 



No increase in Ike absence of oxygen, but an increase in its 

 presence. 



Just the reverse is observed with the primary amido-com- 

 pounds, which decrease by the production of asparagin from 

 them. 



"(2) 



H3)- 



Analytical Data. 



Total nitrogen 



Protein nitr ogcn 



Asparagin nitrogen 



Ammonia 



nitrogen 









u 



2 *e 



0 § 





£ ~i 



O g 





>> i 



O g 



c 









U w <u 



figs 



»i 





Q | g 



1-1 'a 





£ "rt £ 



«-S 



^ CL 













(U 







u 



z 





<U 



y. 



0.465 



9.1 



0.02325 



0.930 



9.2 



O.02288 



0.465 



i-3 



0.00325 



0.465 



0-37 



0.00093 





9-5 







O.i 





») 



1-3 







o.37 





O.461 



9.1 



0.02263 



0.922 



74 



0.01838 



O.46I 



1-7 



0.00431 



0.461 



0-37 



0.00080 





9.0 







7-3 





99 



i-75 







0.27 





O.465 



10.2 



0.02534 



0.930 



8.7 



O.02106 



O.465 



1.4 



0.00353 



0.465 



0-37 



0.000988 





10.15 







8.15 







i-45 







0.42 





