ON THE RESERVE PROTEIN IN PLANTS. II. IQI 



distinguished by treatment with 1 p.m. ammonia, by which the 

 forms of true proteosomes are not changed,* 1 ' while the tonoplast 

 loses its globular shape. (2) 



Another reagent for distinguishing the two kinds of globules 

 is iodine solution, which produces vacuoles in the true proteo- 

 somes, while it only causes the tonoplasts to shrivel up. 



The results of my examinations are shown in the following 

 tables : 



RANUNCULACEj'E. 



Species. 



Objects 

 tested. 



Active 

 albumen. 



Remarks. 



Paeonia albiflora, Pall. 



Old leaves 



Much 



Even present in those 

 living cells that 



Anemone japonica, Sieb. ) 



et Zucc. ) 



Flowers 



None 



were near brown 

 dead portions of 

 the leaves. 



»» i> 



Leaves 



None 





BERBERIDE^E. 



Nandina domestica, Thunb. 



Old leaves 

 Epid. of fruit 



Present 

 Moderate 





TERNSTRCEMIACE^E. 



Camellia japonica, L. 



(Epid. of old! 

 "( leaves j 



Little 



Leaves suffering from 

 albinism also gave 

 the reaction. 



i> », 



Midribs 



Very much 





»> 11 



Epid. of fruit 



11 11 





Camellia Sasanqua, Thunb. 



Epid. of fruit 



Very much 





11 11 n 



Leaves 







11 i> 11 



Flowers 



n 11 





Camellia theifera, Griff. 



Epid. of fruit 



Much 



Small fat globules 



11 11 



Flowers 



Moderate 



present. 



Ternstrcemia japonica, ) 

 Thunb. \ 



Leaves 



Moderate 



Also present in the 

 reddish portion of 

 the leaves. 



(1) They merely solidiy and seem to shrink a little. 



(2) In some cases, however, it is well, after the ammoniacal treatment, to apply 

 100/0 acetic acid under the microscope, which does not affect the solidified proteo- 

 somes, but destroys the globular shape of the tonoplast. I applied this treatment in 

 the case of the petals of Ipomwa hedracea. 



