SUZUKI ; 



a, o.\ % solution of ammonium nitrate. 



b, ,, ,, ,, ammonium chloride. 



c, 0.2 % ,, ,, sodium nitrate. 



d, distilled water. 



Time : — 8 days. (Oct. 27 — Nov. 4.) 

 Temperature :.— Min. io.5°C. ; Max. 40°C. 



Neither the solutions of sodium nitrate, nor the plants grown 

 in the different solutions, gave any reaction for ammonia ; only a 

 few leaves withered during the experiments, and these were re- 

 moved. The result of the analysis was as follows. 



Table I :■ — In 100 parts of dry matter : 



Plants in 



• * x 



OriginaKO plants ControK'-) plants Ammonium Ammonium Sodium 



(Oct. 27th) (Nov. 4th) nitrate chloride nitrate 



Asparagine nitrogen 0.14 0.29 0.78 0.99 o.3g 



AsparagineO) 0.64 1.38 3.67 4.67 1.85 



This result shows that ammonium chloride produced much 

 more asparagine than ammonium nitrate, and this again double as 

 much as sodium nitrate, while an increase of asparagine in the 

 control case, compared with the original plants, taken from the 

 field, must evidently be due to the gradual transformation of 

 nitrates that had been stored up in the stem and roots, whose 

 presence had been shown by the diphenyl-amine test. 



II. Experiments with yellow-lupine (Lupiuus lutcus). 



Young plants 20 cm ' high were taken from the field ; 4-6 

 plants were placed in 300° c - of the following solutions : — - 



a, O.i % solution of urea. 



b, ,, ,, ,, ammonium phosphate. 



c, 0.2% ,, ,, sodium nitrate. 



d, distilled water. 



and kept in the glass house for 6 days, from Nov. 7th to 13th. 



Temperature : — Min. S°C. ; Max. 35°C. 



After drying, the entire plants were used for analysis. 



Table II : — In IOO parts of dry matter. 



(1) "Original" means the plants which were anilyzed at the beginning of the 

 experiments, 



(2) " Control " means the plants kept in distilled water. 



(3) The water of crystallization of asparagine is not included in these calculations. 



