416 



SUZUKI 



Table X : — In too parts of total nitrogen :- 



Plants in 



Original 

 plants. 

 Total nitrogen 100 

 Albuminoid nitrogen 57.3 

 Asparagine nitrogen 17.5 

 Nitrogen in nitrates o 



Control 

 plants. 



Sugar 7 '> Uread) 



IOO 



IOO 



1 : c 



56.5 





53-1 



21 3 



27-4 



45.8 



0 



0 



0 



Sodium 

 nitrate. 



100 



546 



22.4 

 6.0 



Sodium nitrate 

 and sugar. 



IOO 



59.2 

 184 



5-2 



We observe here no considerable decrease of albuminoid ni- 

 trogen during the experiments, but a little increase of asparagine 

 nitrogen takes place in the control and sug.ir case ; but as this 

 increase does not correspond with the decrease of albuminoid 

 nitrogen, it must be due to the transformation of other amido- 

 compounds into asparagine. We observe here also a very great 

 difference of asparagine nitrogen in the urea and sodium nitrate 

 plants. Sodium nitrate does not here seem well suited to be- 

 come asparagine. Sugar had no great influence upon the 

 increase of albuminoid nitrogen ; this must be due to the want 

 of some other necessary conditions for the protein formation 

 (want of sulphates ?). 



Isolation of asparagine crystals : 3.27= rams air dry plants 

 treated with urea yielded o. i 5 ™™ by crystallization, while the 

 calculation requires about o.27 sraui . In this case, I could not 

 separate the slimy mother liquor which prevented the cry- 

 stallization of all the asparagine. 



VII. Experiments with " ktyasag-tira'' {Holesia hispiduvi). 

 Young branches covered with leaves. 25-30 cm- in length, 

 were placed in the following solutions : — 



0.1' 



c, 



% solution of urea. 



and \o% cane sugar. 



0.1% solution of ammonium phosphate, 



and 10 



(3' 



cane 



e, distilled water. 



The objects were kept in the dark, for 7 days (Oct. 31st — 

 Nov. 7th). No ammonia was found stored up after the treat- 

 ment and the leaves remained generally very healthy. 



After drying, the leaves only were subjected to analysis: — 



(1) As the total nitrogen in some of the above cases was increased absolutely, the 

 albuminoid nitrogen was apparently decreased. 



