41 8 



SUZUKI 



The reason why so little asparagine was formed here and 

 the ammonia taken up remained as such partly preserved in the 

 plant, may be due to the fact that the development of young 

 seeds required most of the sugar produced by the leaves, for 

 the production of starch, and therefore the necessary amount 

 of sugar for the production of asparagine was not available. In 

 the following experiments, therefore, all the flowers and seeds 

 were removed before the treatment commenced ; the plants 

 were placed in the following solutions ; — 



a, o. \% solution of ammonium nitrate. 



b, ,, ,, ,, ,, and lo% sugrr. 



c, ,, ,, ,, chloride and io% sugar. 



d, , , ,, ,, carbonate. 



e, ,, ,, ,, ,, and \o% sugar. 

 /, ,, ,, phosphate. 



g, and io% sugar. 



These were kept in the glass house for II days (Oct. 24th — 

 Nov. 4th). 



Temperature: — Min. io.5°c; Max. 37°c. 



In this case, the result differed considerably, no trace of am- 

 monia being found in any of them. 



The analysis for which only the stems and roots were used, 

 yielded the following results. 



Table XIII :— 



Plants treated with 



. Ammo- Ammo- Amm. Ammo- , A mm. 



Ammo- ... , . phos- , , . , 



. niuiiuu- mum carbon- mum l , . chloride 



. , "trate and carbon- ate and phos- P , and 

 trate. . . and 



sugar. ate. sugar, phate. suear. 



& v sugar. & 



Asparagine nitrogen. 0.39 0.10 0.48 0.27 0.26 1.10 0.10 

 Asparagine. 1.82 0.48 2.25 1.27 1.20 0.46 0.50 



We see from these results, that the formation of asparagine 

 from ammonia was considerable. The sugar produced from the 

 leaves evidently yielded the carbon for the asparagine but 

 we see also on the other hand, that, in those cases where sugar 

 was added to the solutions, the amount of asparagine was again 

 less, which finds a natural explanation in the fact that, by a larger 

 excess of sugar, the protein production was so stimulated that 



