422 



SUZUKI ; 



Here also it is seen that the increase of asparagine nitrogen 

 is accompanied with the decrease of nitrates in the plants, and 

 that in this case the asparagine is not a decomposition product of 

 proteids. 



The relation of these numbers will become still clearer, if 

 we add the following table : — 



Table XIX. In 100 parts of total nitrogen. 



Plants treated with 



( )riginal 

 plants 

 IOO 



35-9 

 69 

 22.7 



Control 

 plants 

 IOO 



36.7 



16.0 

 8.1 



Ammonium 

 chloride 

 ioo' 1 ) 

 42.9 

 20.0 

 14-3 



Sodium 

 nitrate 

 IOOW 

 32 2 

 22.5 

 21.0 



Total nitrogen 

 Albuminoid nitrogen 

 Asparagine nitrogen 

 Nitrogen in nitrates 



III. Experiment. 



Here, the influence of sugar and that of temperature upon 

 the result was to be observed. The plants were taken from the 

 same farm as before, and placed in glass cylinders, containing 

 each 500 C-Cl of the following solutions : — 

 10% solution of sugar. 



/a. 



b. 



c. 



d. 



e. 

 If. 



0.2 ' 



ammonium chloride. 



sodium nitrate. 



and 10% sugar. 



and 10% sugar. 



distilled water. 



g. 10% sugar solution. 



distilled water. 



a, b, c, d, e, f, were kept in the glass house, while g, and h, 

 were kept in the laboratory, where the temperature was much 

 lower than in the glass house. (2) 



Duration of experiment, 8 days (Dec. 10th — 18th). 

 Temperature in the glass house :— Min. i°C ; Max. 38°C. 



,, ,, laboratory :— Min. 5°C ; Max. 1 5°C. 



Plants treated 

 with 



Total nitrogen absorbed 



in grams 

 Percentage of absorbed 

 nitrogen in the dry 

 matterW 



Ammonium 

 chloride 



0.042 



°-53% 



Ammonium chloride 

 and sugar 



0.31% 



Sodium 

 nitrate 



0.041 



o.54% 



Sodium nitrate 

 and sugar 



0.037 

 o-34% 



(1) The nitrogen absorbed during experiments was subtracted from the total 

 nitrogen and the remaining number ( = total N. before experiment) was calculated as 100. 



(2) Toward the end of the experiment, the plants kept in the sugar solution in the 

 glass house began to suffer. The top of the leaves, dried and turned yellow, but those 

 kept in the cooler laboratory remained healthy. 



(3) Total dry matter of the plants treated with ammonium chloride = 7.901 grams. 



,, „ „ „ sodium nitrate = 7619 „ 



„ „ „ ammonium chloride and sugar = 12.393 „ 



„ „ ,, sodium nitrate and sugar = 10 936 „ 



