FORMATION OF ASPARAGINE IN PLANTS. 423 



After careful washing and drying the entire, plants were 

 analyzed. 



Table XX. In 100 parts of dry matter : — 





Temp. 1-3SX'. 



Temp. 

 S~*5°C- 









Plants 



treated with. 





























( (riginal 

 plants 



Control 

 plants. 



Sugar 



\ mmoniun 

 chloride 



mm. chloiii 

 and sugar 



Sodium 

 nitrate 



rt u 

 ~ - 



g « 



Control 

 plants 



Plants in 

 sugar 













< 





■j} 







Total nitrogen 



428 



3-64 



248 



4.07 



2.94 



5°o 



2.81 



431 



2.63 



Albuminoid nitrogen 



1 98 



1.42 



1-37 



• I.76 



1.52 



1 75 



i-3« 



2 13 



1.64 



Asparagine nitrogenO) ... 



0 40 



0.72 



0 18 



099 



0.27 



0.82 



021 



048 



0.22 



Nitrogen in nitrates 



0.43 



009 



0 



o.^3 



0 



0.76 



0.14 



0.16 



0 



As the dry matter had increased very much in those plants 

 which were treated with sugar, the percentage of the nitrogen 

 compounds is very much lowered. I calculated therefore the fol- 

 lowing table to show the relation more clearly : — 



Table XXI. In 100 parts of total nitrogen : — 





Temp. i-38°C. 



Temp. 

 5-i5°C 







Plants treated 



with ;- 

















•> 

 -a 



■r* i_ 





V 



2 * 









Original 

 plants 



Control 

 plants 



u 



rt 



3 

 J. 



mmoniu 

 chloride 



m. chloi 

 nd suga 



Sodium 

 nitrate 



-3 rt 

 - 3. SO 

 ~ 3 



3 t/i 



Is 



Control 

 plants 



Plants ir 

 sugar 











< 



E rt 



















< 





■n 







Total nitrogen 



100 



100 



IOO 



100W 



100W 



IOO< J ) 





IOO 



IOO 



Albuminoid nitrogen 



463 



38.1 



55-0 



49 7 



57.8 



39 2 



530 



49-4 



62.4 



Asparagine nitrogen 



93 



19.8 



7-3 



28.0 



103 



18.4 



8.2 



1 1.1 



8.4 



Nitrogen in nitrates 



11. 5 



2-5 



0 



6.5 



0 



17.0 



57 



3-7 



O 



(1) Asparagine nitrogen was here determined without previous removal of the bases 

 by phospho-tungstic acid; therefore the result is somewhat too high. 



(2) For the calculation the absorbed nitrogen was subtracted from the total nitrogen; 

 otherwise a true comparison is impossible. 



