426 



SUZUKI ; 



of dilute dammonia. The concentrated solution yielded an abund- 

 ant quantity of large asparagine crystals which were washed 

 with water and then with absolute alcohol, and dried over sul- 

 phuric acid. The amount thus obtained was 0.51 gram, (1) the an- 

 alysis proved the crystals to be pure asparagine. 



Calculated Observed 



Nitrogen 18.66% 18.45 % 



Water of crystallization. . . 12.0 % 12.2 % 

 (lost at I00°C.) 

 XI. Experiment with barley (Hordeum disticJion). 

 Young barley plants of an average hight of 32 cm - were removed 

 from field and placed (in the glass house) in the following solu- 

 tions : — 



urea. 



a. 

 b. 

 c. 

 d. 



e. 



0.2 



and 2 % sugar. 



,, ammonium chloride. 



and 2 % sugar. 



2 % sugar, 

 f. distilled water. 

 Duration of experiment : — 7 days (March 26th — April 2nd). 

 Temperature : — Min. iO°C. ; Max 45 C C. 



After drying, the entire plants r2) were analyzed with the 

 following result : — 



Table XXIV. In 100 parts of dry matter : — 



Plants treated with 



Original 



Control 



Sugar 



Urea Urea and 



Amm. 



Amm. chloride 



plants 



plants 



sugar 



chloride 



and sugar 



Total nitrogen 5.13 



5- 08 



4.60 



5 65 4-98 



5-73 



5.00 



Alb. nitrogen 2.16 



1.86 



1.47 



1.50 1.28 



1.60 



1 23 



Asp. nitrogen 0.93 



1.84 



1.94 



i.87(?) 2.15 



3.21 



2.09 



Nitrate nitrogen 0.58 



o.37 



o.35 



0.49 0.24 



0.28 



0.23 



Table XXV. I 



n 100 parts of total nitrogen : — 











Hants treated with 





Original 



Control 



Sugar 



Urea Urea and 



Amm. 



Amm. chloride 



plants 



plants 



sugar 



chloride 



and sugar 



Total nitrogen 100 



100 



100 



100 100 



IOO 



100 



Alb. nitrogen 42.3 



36.6 



32 



26.6 25.7 



27.9 



24.6 



Asp. nitrogen 18. 1 



36.2 



42.2 



33.i(?) 43.2 



55-8 



41.8 



Nitrate nitrogen 1 1 .4 



7-3 



7.6 



8.6 4.8 



4-9 



4.6 



(1) A moderate quantity of asparagine was left in the mother liquor, which was not 

 considered. 



(2) Toward the end of the experiments some leaves or tips of leaves began to dry 

 up, owing to the high temperature. Such leaves were, of course, removed. 



