FORMATION OF ASPARAGINE IN PLANTS. 



429 



ANALYTICAL DATA. 



For the determination of total nitrogen cited in the fore- 

 going pages 0.5 or i.o sram of air dry sample was mixed with 30 c c - 

 sulphuric acid, i siam sugar, 2 grams benzoic acid, i sram mercury 

 and o.5 Bram copper sulphate, and decomposed and distilled as 

 usual. 



For the determination of proteid nitrogen, Stutter s method 

 was used. 



For the determination of asparagine nitrogen Sacchsse's 

 method was generally used, but in some cases, when the presence 

 of organic bases was suspected, phospho-tungstic acid was pre- 

 viously applied. 



For the determination of nitrates, Tietnann and Sc/mltze's 

 method was used. 



HELIANTHUS ANNUUS. 



ASPARAGINE NITROGEN. 



Plants in 



Dry matter 

 used in 

 grams. 



BarytaU) 

 water re- 

 placed in c.c. 



Nitrogen 

 found in 

 gram. 



( i- 4 535 



18.8 



0.0178 



[2. 



18.6 



0.0176 



j 1. 448S 



23-7 



0.0224 



u „ 



23-4 



0.0221 



j l. 4-550 



9-4 



0.00S9 





9-4 



0.0066 





7.0 



0.0064 



1 4 :: s 



6.8 



0.0030 





3-2 



0.0029 



1 1. 4405 



3-i 



0.0029 



Percentage 

 of nitrogen 

 (=£ asp. 

 nitrogen.) 



Ammonium nitrate . 

 Ammonium chloride 



Sodium nitrate 



Control plants 



Original plants 



039 



0.50 



0.20 



0.15 



0.07 



(1) 10 c.c. of the standard sulphuric acid were equivalent to 48 c.c. baryta solution, 

 and yielded 0.374 gram barium sulphate. Hence ice. of the baryta solution = 0.0009447 

 gram nitrogen. 



