FORMATION OF ASPARAGINE IN PLANTS. 437 



HOLESIA HISPIDUM. 



ASPARAGINE NITROGEN. 



Plants in 



Dry matter 

 used. 



Baryta waterW 

 replaced. 



Nitrogen 

 found. 



Percentage of 

 nitrogen (J asp. 

 nitrogen.) 



Ammonium phosphate 



I 1 " 



u 



Gram. 

 3.664 



c.c. 

 3° 



3° 



Gram. 

 I 0.0028 



0.08 



Ammonium phosphate 

 and sugar 





.2- 



3724 



1.9 



2,1 





0.0019 



0.05 



Urea 



I 1 - 



u 



3.668 



4.2 



4-5 





0.0042 



O.I I 



Urea and sugar 



_ 



2. 



1.790 



10 

 1.2 





0.0010 



0.06 









3.692 



30 

 3-o 







Control plants 





* 



|- 0.0028 



0.08 



Original plants 







2.601 

 »» 



i-5 



1.6 



1 0.0015 



0.06 



BUCKWHEAT. (I.) 



DETERMINATION OF AMMONIA. 



One gram of air dry matter of the ammonium nitrate plant 

 (lower stems and roots) was extracted with warm water and to 

 the filtrate, to which so much sodium bicarbonate added as 

 necessary to render the liquid slightly alkaline, yielded on 

 distillation 0.0017 gram nitrogen (=i.8cc. baryta solution' 1 ') 

 = 0.17% nitrogen. 



Air dry samples were mixed with some caustic lime and 

 moistened with water. The mixture was kept under a bell jar. 

 The ammonia liberated was absorbed by a known quantity of 

 standard sulphuric acid ; thus I obtained the following results: — 



1 gram of air dry sample of the control plants yielded 

 O.0004 gram nitrogen (=o.4cc. baryta solution (1) ) = o.04 < %' nitrogen. 



(i) 1 c.c. of the baryta solution = 0.0009447 gram nitrogen. 



