FORMATION OF ASPARAGINE IN PLANTS. 441 



NITRATE NITROGEN. 





Dry matter 

 used. 



Pressure. 



Tempera- 

 ture. 



Volume of 

 NO gas. 



Nitrogen 

 found. 



Percentage 

 of 



nitrogen. 





Gram. 



Inch. 



°c. 



c. c. 



Gram. 





Control plants ... 



| 1. 0.913 



3°-3 6 



9 



11.9 

 12.1 



| 0.0076 



0.83 



Original plants .. 



| I- 0.917 







6.0 

 6.0 



| 0.0038 



0.41 



BRASSICA. (TAINA). (I.) 



ASPARAGINE NITROGEN. 



Plants in 



Dry matter 

 used. 



Baryta waterO 

 replaced. 



Nitrogen 

 found. 



Percentage of 



nitrogen 

 Q asparagine 

 nitrogen.) 





Gram. 



c. c. 



Gram. 





Ammonium phosphate 



1.840 



2.0 



0.0043 



0.23 



Sodium nitrate 



I.830 



1.8 



0.0039 



0.21 



Control plants 



1.858 



1.7 



0.0037 



0.20 



BRASSICA. (TAINA). (II.) 



I. Nitrogen determination of ammonium chloride solution 

 applied to the plant (before experiments). 5°- c - of the ammonium 

 chloride solution were diluted with water to 250 ce> , and from 

 this 20^- and icxf'^ were distilled after addition of soda solu- 

 tions. 



1. 2O 0C ' yielded 0.0160 gram nitrogen ( = i6.c/- c - baryta 

 solution (1) ) = o.o8 - % nitrogen. 



2. ioo 00- yielded 0.0792 gram nitrogen ( = 83.8 0c - baryta 

 solution) =0.08% nitrogen. 



This calculated to the original solution gives 

 I. 3-99% 



2- Average 3.98%". 



(1) 1 c.c. baryta solution =0.0009447 gram nitrogen. 



