FORMATION OF ASPARAGINE IN PLANTS. 



445 



BRASSICA CAMPESTRIS VAR. TAINA. (III.) 



I. Examination of ammonium chloride solution that remained 

 unabsorbed. The total solutions were diluted to 5OO C0- , of 

 which 200 c-c - and ioo cc - were analyzed. 



(a) . 200 c-c ' of the ammonium chloride solution yielded 



0.0627 g ram nitrogen ( = 66.3 CC- baryta solution). 

 Hence total nitrogen in total solution = 0.1 568 gram. 



(b) . ioo c c - of the ammonium chloride and sugar solution yielded 



O.0321 gram nitrogen ( = 34 cc- baryta solution). 



Hence total nitrogen in total solution = o. 1606 gram. 



Total nitrogen originally applied = 0.1988 gram. 



Therefore, 0.1988 — 0.1568 = 0.042 gram nitrogen was absorbed by 

 ammonium chloride plants. 



0.1958 — 0.1606=0.0382 gram nitrogen was absorbed 

 by ammonium chloride and sugar plants. 



II. Examination of sodium nitrate solution that remained 

 unabsorbed. The total solutions were concentrated to ioo 00- , 

 from which 20 and io c,c - were analyzed. 



(a) . 20 1 " 0, of sodium nitrate yielded 



0.0218 gram (temp. I5°C, pressure 29.66 inch., vol. of 

 NO 36.4 ;'•«•). 



Total nitrogen in total solution = 0.1090 gram. 



(b) . I0 C C - of sodium nitrate and sugar solution yielded 



0.0113 gram nitrogen (temp. I5 C C ; pressure 30 inches ; vol. 

 of NO i8.5 cc -). 

 Total nitrogen in total solution = 0. 1 126 gram. 

 Total nitrogen originally applied = 0.1499 gram nitrogen. 



0.1499 — 0.1090 = 0.0409 gram nitrogen was absorbed by 

 sodium nitrate plants. 



0.1499 — 0.1126 = 0.0373 gram nitrogen was absorbed by 

 sodium nitrate and sugar plants. 



