NAKAMURA ; ON ASPARAGINE AS A 



NUTRIENT FOR FUNGI. 469 



TABLE I. 



Nourishing material in grams, for 500 c. c. 



\\ eight of fungus in grams. 



Ammonium tartrate 6.33 4- ethyl alcohol 3-^6 



0.015 



,, malate 5.00 + ,, ,, ., 



0.01 1 



succinate 5 00 -4- ,. >> 



0.008 



„ lactate 7.13 + ,, „ „ 



0.006 



„ acetate 5.13 4- „ „ „ 



O.000 



„ nitrate 2.66 + „ „ „ 



0.002 



Asparagine 5.00 + „ ,, ,. 



0.026 



,, 5.00 alone 



0.016 



Second exjoeriment. 



Here methyl alcohol was applied in addition to the nitro- 

 genous materials which were asparagine, glycocoll, urea, betain, 

 ammonium chloride, sodium nitrate, ammonium tartrate, am- 

 monium malate, and ammonium succinate. All the solutions 

 contained 1 °/o methyl alcohol, while the sources of nitrogen were 

 applied in such proportion that the number of nitrogen atoms to 

 those of carbon showed the ratio of 1 : 8. This time the amount 

 of monopotassium phosphate was increased to 0.5 % an d to all the 

 flasks ferrous sulphate, (1) sodium sulphate, and magnesium sulphate 

 (o. 1 °/o °f each) were added. All other conditions were the same 

 as before except that the volume of the solutions was only 200 c.c. 

 and the temperature somewhat higher, ranging from 9 0 — ig°C The 

 flasks, after two weeks, exhibited a considerable difference; in be- 

 tai'n, ammonium succinate and ammonium malate, no fungoid de- 

 velopment was yet noticcd (2) ; in ammonium tartrate only a moder- 

 ate quantity was observed, much however in asparagine and glyco- 

 coll. The flasks had been frequently shaken in order to prevent 

 the formation of spores on the surface which would have again 

 given rise to a great increase of the mycelium. Only during the 

 last 4 days shaking was dispensed with, allowing now spores to 



( 1) Certain fungi are only developed in presence of iron salts (Molisch) ; according 

 to Raulin also presence of zinc salts will promote the development of mould fungi. 



(2) They were infected again and left to stand for several months, whereupon a 

 moderate development was observed. 



