492 



MAE NO 



10 cc. of the diluted yeast applied corresponded to 0.0613 

 gram dry matter. 



After 5 days' fermentation, the mixtures were filtered off and 

 on the one hand, the dry matter of the yeast, on the other, the 

 amount of sugar still present were determined. 



The yeast A weighted 0.165 S Tam > the yeast B 0.198 gram. 

 The volumetrical sugar determination still showed in the flask A 

 3.52 gram, in B, however, only 3.07 gram, of sugar. 



A B 



Increase of yeast in grams 



0.1037 



0.1367 



Increase of yeast in percentages of dry yeast. 



169 %. 



223 %■ 



Sugar fermented in grams 



3-3360 



37860 



Sugar fermented in percentages of dry sugar. 



48.80 %. 



55-2o %. 



We see, therefore, that the amidosulphonic acid does not 

 prevent the growth and fermentative power of the yeast, but it is 

 a less favourable source of nitrogen than ammonium sulphate, 

 which again is a surprising fact, as the chemically powerful yeast- 

 cells should in our opinion be capable of easily bringing on the 

 hydrolysis of the amidosulphonic acid. 



VI. Experiments with Mammalia. 



I made in this regard but few experiments. Into a white 

 mouse was injected subcutaneously 0.5 cc. of a 1 °/o solution of 

 sodium amidosulphonate. Since I could not observe any noxious 

 effects after 48 hours, I injected once more 1 cc. of the same 

 solution. Soon afterwards a considerable increase of the respira- 

 tory activity was noticed, but 2 days later the mouse was in a 

 normal state again. 0. 



In another experiment, I soaked bread in a 1 % solution of 

 sodium amidosulphonate and fed a mouse with it. This animal 

 became gradually very weak and somnolent and died after 

 76 hours. In this case perhaps such a large quantity of the 



(I) Tiiis result agrees with those obtained by Prof. Takahashi in the Imperial 

 University in Tokyo. The observations of Prof. O. Loew on lower aquatic animals, 

 ■which remained alive in 1 p. m. solution of calcium amidosulphonate, are also in accord- 

 ance with my results. 



