508 



SHIMADA 



Upon dilution with water, boiling with barium carbonate, 

 evaporation of the filtrate and extraction with alcohol containing 

 some ammonia, a characteristic crystallisation of leucine and 

 tyrosine was obtained. The leucine was also recognised by the 

 odour of amylamine on heating, while it showed tyrosine by 

 Millori s reaction. 



Whether also arginine, lysine, aspartic acid, glutamic acid 

 and phenyl-amido-propionic acid was formed, I hope to decide 

 later with larger quantities. 



For analysis the product was dried at ioo° C. 

 I. 0.230 grm. gave 0.4863 grm. C0 2 and 0.149 S vm - H 2 0. 



= 57,65 %C and 7.2 %H. 

 II. 0.1636 grm. gave 0.3500 grm. C0 2 and 0.103 S rm - H.O. 



= 5^-34 % C and 7.0 % H. 

 I. 0.5000 grm. gave after Kjeldahrs method 

 68.03 mg. N= 13.60% N. 

 II. 0.5000 grm. gave 67.22 mg. N= 13.45 % N. 0 '' 

 I. 1. 0000 grm. substance gave after heating with a mixture 

 of sodium carbonate and some potassium chlorate 0.117 grm. 

 BaS0 4 =i.6o% S. 



II. 1. 0000 grm. yielded by the same method 0.090 grm. 

 BaS0 4 =i. 3 o^S. 



These results would correspond approximately to an albumin 

 in which three hydrogen atoms have been replaced by three phenyl 

 groups, when Lieberkiihri s formula is taken as a foundation. 



The product must then have been formed according to the 

 following equation : — 



C 72 H 112 N 18 SO 22 +3(C 6 H 5 OH)=C 72 H 109 (C 6 H 5 j s N 18 SO :B +3H 1 O. 





Theory. 



Experiment. 



I. 







II. 



c 



58.69 



57.6s 







58.34 



H 



6.74 



7.20 







7.00 



N 



I3-70 



13.60 







1345 



S 



1.74 



1.60 







1.30 



O 



i9-!3 













100.00 











(I) Another sample yielded Str.uki, of this College, 14.44% nitrogen. 



