186 



Appendices to Fourth Annual Report 



deeper where the growth is thickest, ?.e., near the centre, and lighter at 

 the margins. The mass as a whole is moist, but it is covered with a 

 delicate dry film. Slightly mamillated. 



Microscopically — Ovoid micrococcus. Many partially divided, resem- 

 bling short rods, but nearly all can be resolved. "4 /a broad and "6 to 

 *65 /X long. 



17. A beautiful moist sulphur or cadmium-yellow growth of consider- 

 able thickness, which spreads pretty rapidly over the surface of the bread 

 paste, and has a number of small mamillated projections above the general 

 surface of the growth. 



Microscopically — A torula, the cells of which spherical, ovoid, or 

 oblong, averaging '8 jx in transverse diameter and 1 '4: /u, in length. 



18. In a large number of flasks there is a flesh-coloured very watery 

 mass, which grows with extreme rapidity. It spreads rapidly at the 

 margin, and may attain a considerable thickness. When it dries at the 

 margins other forms do not encroach upon it. 



Microscopically, it is a slender bacillus, -3 to '4 /w, in diameter and 

 1'2 fx in length, forming dense masses of filaments, with some round 

 spores '4 in diameter, the formation of which is well seen. Differs 

 from No. 11 (yellow bacillus) in the much more hollow character of its 

 filaments, the hyaline masses which they form, and the mode of spore 

 formation. 



19. A second very similar but somewhat drier flesh-coloured mass was 

 also found. 



It consisted (microscopically) of very minute bacteria '2 to '3 /x in 

 diameter, and 1 -3 to 2 yu, long, packed in dense masses. No longer fila- 

 ments seen. 



20. A third flesh-coloured growth consists of oblong micrococci, mostly 

 in pairs -5 /a in diameter and "8 to 1 ju, in length, or actively dividing. 



21. There is also another yellow growth, somewhat similar in its naked 

 eye appearances to No. 8, but microscopically it is seen to consist of very 

 minute delicate bacilli, -2 to '25 ^ wide and '8 to 1 long. The 

 outlines are very faint, and distinct spore formation may be seen. The 

 rods are straight or slightly curved. 



22. Light brown, moist growth. 



Microscopically — Short solid rods with rounded ends, very various in 

 length, 1 to 2 /A, and about "5 ju, broad. Seen to be dividing actively, 

 but with no spore^formation. 



23. A pink growth found in some of the flasks, almost like pink coral, 

 growing on the surface of the bread paste. It is delicate rose-pink, dry 

 or moist according to the medium on which it is inoculated. It may 

 attain a considerable thickness, when the margins are usually scalloped. 



In gelatine the characteristic pink growth is found only at the free 

 surface. Along the track of the needle the growth is less luxuriant and 

 is dull grey, in which a very delicate pink shade may be made out. It 

 does not liquefy gelatine. 



Microscopically — A torula, the cells of which are '6 yu. to 1 or 1 '25 fx 

 in diameter. 



24. There is also a yellow torula growing in some of the flasks. In this 

 form the cells are more rounded, and measure only "5 to "65 /u, in diameter. 



25. One of the most common if not the most common of the putre- 

 factive bacteria appears as a glistening grey layer on the surface of the 

 bread paste, on which it grows with extreme rapidity. This moist grey 

 layer gradually becomes darker in colour. When inoculated into gelatine 

 it liquefies it very rapidly, especially at the upper part of the track of the 

 needle. A funnel-shaped depression is formed containing a slightly turbid 



