of the Fishery Board for Scotland. 



187 



fluid tinged with green. Gradually the upper layers of gelatine are com- 

 pletely liquefied, and the liquid is of a more distinctly green colour, which 

 later becomes deep citron yellow. Growing on bread paste, it gives rise 

 to a strong putrefactive odour. 



Microscopically — "5 to '7 broad and 1*5 long. Bacterium termo. 



26. A very pale, almost (oil paint) white, opaque growth, which ex- 

 tends over and through the bread paste, making its appearance on the 

 under surface, discolouring it somewhat. On the surface of this growth 

 there is formed after a time a darker, thin, dry, wrinkled film, which 

 gradually becomes thrown into folds. This growth spreads rapidly over 

 the whole surface of the bread paste, and ultimately obscures most other 

 forms which may have started with it. 



Inoculated into nutrient gelatine an opaque white line is seen along the 

 track of the needle. Liquefaction commences at the surface, and slowly 

 following this track works downwards, a thick, dull white precipitate being 

 deposited at the bottom of the liquefied portion, which is usually slightly 

 opaque. When a drop of water has been shaken up with the nutrient 

 jelly and left in the test tube, the presence of this same organism may be 

 suspected when we see developed small, clear, rounded spaces in the jelly, 

 with a dull white deposit at the lowest part of the globule. It causes 

 liquefaction of the jelly more slowly than bacterium termo (No. 22). 



Microscopically — A bacillus about 2 /u, in diameter and from 6 to 

 8 /A long. Sometimes seen in longer threads. Distinct spore forma- 

 tion, the spores appearing at smaller parts of the rods. 



Tay. 



The following are the most important species which have been isolated 

 from Tay water : — 



1. A brilliant moist cadmium yellow growth which spreads somewhat 

 rapidly over the surface of the bread paste, especially if the surface be 

 moist. At the point of inoculation the growth is thickest, and at the 

 centre of the growth the bread paste is completely covered, but nearer the 

 margins the yellow growth runs in the fissures or depressions where the 

 moisture is collected. 



In gelatine, on the third day, there is a grey point of growth on the 

 surface, but as yet no liquefaction. On the seventh day it has assumed a 

 yellow tinge, and the gelatine is slightly softened, allowing the surface 

 growth to sink into a kind of cup. 



Microscopical Characters — It consists of rounded cells, single or double 

 or budding, '7 to '8 fx in diameter and -8 to \ fx in length. A yellow 

 torula. 



2. Light brown mass, growing as smooth light brown or fawn-coloured 

 elevated patches on the surface, of which there is some slight roughening. 

 Some of these patches grow directly on the bread paste, but others are 

 seen on the surface of the yellow growth above-mentioned that occurs in 

 the same flask. 



When inoculated with gelatine it does not give rise to liquefaction at 

 the end of seven days. There is simply a small pearly-grey growth at the 

 surface, somewhat thickened at the margins, which are scalloped. A very 

 delicate growth along the track of the needle. 



Microscopically — A minute rounded micrococcus '35 to '4 /a in diameter. 



3. White oil paint mass mamillated with pure white points growing on 

 the surface. Surface smooth and glistening, but dry. 



