of the Fishery Board for Scotland. 



23 



38 days. — A dozen eggs taken off one of these plates included three live 

 eggs. The heart was beating slowly. . The eyes were beginning to show 

 ome black pigment. One egg exhibited the condition where the tail 

 nearly reaches the head, I saw no crystals inside the zona. 



JfJf days. — Of 38 dead eggs, 19 contained embryos ; of the embryos, 7 had 

 pigmented eyes. 



50 days. — The cooling ceased on this date ; no larva had hatched out. 

 The plates were transferred to tank No. 2. 



52 and 53 days. — No live egg was found. Some eggs had died in the 

 disc stage, but great numbers have embryos of greater or less size. A good 

 number of the eggs next the glass where the ova have been two or more 

 layers thick had died in the disc stage. 



None of the eggs of these plates had hatched. 



Plate 19. 

 4 days.- -The eggs looked very well. 



8 days. —The eggs were alright ; some at the margin were dead. 



9 days. — This plate did not show many dead eggs ; it was in better con- 

 dition than those in frame B. 



12 days. — Some dead eggs were noticed. 



20 days. — There was a largo quantity of eggs dead. The live eggs were 

 found all over the plate ; the dead eggs were massed in the central region, 

 where there are also a few live eggs. The dead eggs were mainly where the 

 eggs were two layers deep. In one case some eggs on top were dead, while 

 those next the glass were alive. Some eggs showed a fairly long tail, which 

 did not, however, reach to the head. I could see crystals in some eggs, none 

 in others. 



28 days — The majority of the eggs were dead, but numbers were still 

 alive ; one was in the stage similar to that found 8 days previously. Nume- 

 rous infusors were running over the live eggs. 



51 days. — I did not notice a single live egg. On one part of the plate 

 where the eggs formed an uniform single-layered stratum completely covering 

 the portion, I found that the dead eggs had been pretty well developed. 

 Inside a high heap the eggs had died early when the embryo was forming. 

 The eggs were well covered in parts with sporozoan colonies and fungus. 



