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Part III. — Sixteenth Annioal Report 



Fig. 11. — Section of advanced ovum of Angler, 0'9mm. in diameter, from the 

 ovary of a fish, 48 inches long, caught on 9th February. Preserved in picro- 

 sulphuric acid, and stained with hsematoxylin, safFranin, and eosin. a, Menibrana 

 propria foUiculi. 



Fig. 12. — Section of eggs from the ovary of an Angler, 45 inches in length, caught 

 on 20th May, showing yolk-nucleus in two contiguous eggs. Preserved in Perennyi's 

 fluid, stained as before. 



Fig. 13. — Section from the ovary shown in figs. 1 to 4, showing the mucoid secretion, 

 the partial fusion of the yolk-spherules in the almost ripe eggs, &c. ; g.v.^ germinal 

 vesicle in process of disintegration (see fig. 4, Plate III.), the nucleoli breaking up 

 into granules; y, yolk partially fused ; m, mucoid secretion; s.e., mucin-secreting 

 epithelium ; w.o., greatly attenuated wall of ovary ; d.o., degenerating ova at base 

 of pouches; /o., follicle. Preserved in picro-sulphuric acid, stained with 

 hsematoxylin, safiranin, and eosin. 



Fig. 14. — Section of ovary from which Fig. 11 is taken, showing the arrangement 

 of ovigerous pouch ; a, large ovum represented in outline ; h, base of pouch showing 

 the upward protrusion of sub-epithelial tissue; ?y.o. , w^all of ovary; s,e., mucin- 

 secreting epithelium. 



Plate III. 



Fig. 1. — Section of ovary of Angler showing arrangement of pouches, from some- 

 what stale material ; preserved in picro-sulphuric acid, stained with borax -carmine. 

 Magnified 50 times, a, Columnar epithelium. 



Fig. 2. — Small fresh egg of Zeus faher, showing the peri-nuclear zone of oil-globules. 



Fig. 3. — From the same sections of ovary of Angler as Fig. 11, representing the 

 epithelial cells near the base of a pouch. Zeiss 2mm. oil immersion, comp. oc. 4. 



Fig. 4. — Germinal vesicle of the central ovum in the Angler in fig. 13, Plate II., 

 showing its disintegration at the inner surface of zona radiata, fusion of contents 

 with scanty cortical layer of protoplasm, and the vacuolation and breaking down of 

 the nucleoli into spherical granules. 2mm. Zeiss oil immersion, comp. oc. 4. 



Fig. 5. — Germinal vesicle of ovum of Angler, represented in fig. 11, Plate 11. , 

 showing the disintegration of the nucleoli, the chromosomes or tangle in the centre, 

 and chromatin granules dispersed throughout its substance. a, Nucleolar shell ; 

 h, granular bodies. 2mm. Zeiss immersion, comp. oc. 6. 



Fig. 6. — Germinal vesicle of egg of Angler, from section shown in fig. 12, Plate 

 II. 2mm. Zeiss immersion, comp. oc. 4. 



Fig. 7. — Peripheral portion of germinal vesicle of an egg of Angler, from same 

 ovary as fig. 4, showing breaking down of nucleoli, the chromosomes or tangle, and free 

 chromatic granules, stained with eosin, rubin, and orange. 2mm. Zeiss, comp. oc. 6. 



Fig. 8. — Ovaries of Zeus faher. Reduced. 



Fig. 9.— Section of egg of same, showing fused oil vacuoles and granulated 

 cytoplasm. Preserved in picro-sulphuric acid, stained with hsematoxylin, saffranin, 

 and eosin. 



Fig. 10. — Larger egg of same. 



Fig. 11. — Egg of same, showing oil vacuoles, yolk-spherules, and layers of 

 capsule. 



Fig. 11a. — Yolk-spherules of same. 



Fig. 12. — Germinal vesicle of ovum represented in last figure, showing chromo- 

 somes. 



Fig. 13. — Section of ovary of same, showing capsule and arrangement of eggs. 



Fig. 14. — Germinal vesicle of egg, same size as fig. 10, showing chromosomes 

 nucleoli, and free chromatic granules. 2mm. Zeiss oc. 4. 



