DIGESTIVE ENZYMES IN POIKILOTHERMAL VERTEBRATES 195 
Table 11. — Amylase in pancreas of snapping turtle 
[Digest=0.5 c. c. extract in 50 c. c. total (1 per cent starch, 5 per cent toluol)] 
Description 
Minutes 
Net gain 
in 60 
minutes 
0 
5 
10 
15 
30 
60 
Milligrams glucose per cubic centimeter digest - - 
1.11 
Blue. 
2. 86 
Lighter 
blue. 
4.00 
Lavender. 
5.00 
6. 55 
Very light 
blue. 
6. 25 
Nearly^ol- 
orless. 
5. 14 
Table 12. — Amylase in pancreas of painted turtle 
[Digestion at 24° C. and at 37° C. The (25 per cent tissue) extract was diluted to one-tenth strength. Digests=l c. c. diluted 
extract in 60c. c. total (1 per cent starch, 5 per cent toluol). Equivalent to the extract from 1 gram of pancreas to 4,000 e. c. 
1 per cent starch] 
Description 
Minutes 
Net gain in 
60 minutes 
0 
30 
60 
Temperature 24° C: 
Reducing power in milligrams glucose per cubic centimeter digest 
Color of iodine test _ 
1. 11 
Deep blue. 
1. 11 
Deep blue. 
2,08 
Blue. 
2. 13 
Blue. 
3. 12 
Purplish. 
3. 33 
Purplish. 
2.01 
2.22 
Temperature 37° C: 
Reducing power in milligrams glucose per cubic centimeter digest 
Color of iodine test 
INVERTING ENZYMES 
Tables 13 and 14 show tests made for the presence of invertase. With the 
exception of the bluegill, the caeca of which were used, and the pickerel, of which 
the entire digestive tract was tested, investigation for invertase was limited to the 
intestine. In the carp different regions of the entire alimentary canal, which shows 
characteristics of an intestine, were tested. In the bluegill some invertase was 
found in both the caeca and the intestine. No invertase was found in the small 
intestine of Necturus, the bull snake, or the large intestine of the snapping turtle. 
It was present in a very small amount in the intestine of the pickerel. The carp, 
bluegill, and snapping turtle each possessed a relatively large amount of invertase. 
It is an interesting fact that these three animals include a considerable amount of 
vegetable food in their diet. The other three animals in which invertase was absent 
or present only as a slight trace feed almost wholly on animal food. 
