Vol.. 7, 1921 
PHYSIOLOGY: OSBORNE AND MENDEL 
161 
tein — casein, edestin, or lean beef which had been thoroughly extracted 
with boiling water — inorganic salts, agar-agar, lard, butter fat and 0.4 
gm. daily of dried brewery yeast furnishing vitamin B. This amount 
of yeast can scarcely be regarded as a significant source of available carbo- 
hydrate. Success was likewise attained in experiments in which no 
agar-agar was introduced. In the latter case the only obvious sources 
of preformed carbohydrate aside from the yeast employed were such 
carbohydrate impurities as might still adhere to the protein preparation 
fed. 
The newest experiments conducted with materials from which the 
carbohydrate was even more rigorously excluded have not yet been 
brought to a conclusion ; but in these also considerable growth at a normal 
rate has already been attained. It will be observed that the dietary con- 
ditions under which this series of nutrition experiments has been conducted 
are such as are reputed to lead to ketosis and ketonuria in human subjects. 
280 
240 
200 
160 
120 
80 
40 
GROWTH ON DIETS IN WHICH NEARLY ALL 
OP THE ENERGY WAS SUPPLIER BY 
PROTEIN 
Casein 
Zein 5 
Salt mixture 5 
Alfalfa 0.4 g. daily 
Yeast 0.2 g. daily 
40 days > 
Meat residue 
Gliadin 
Salt mixture 
90% 
5 
5 
Alfalfa 0.4 g. daily 
Yeast 0.2 g. daily 
CHART 3 
