PHYSIOLOGY: L. S. PALMER 
587 
materials commonly used as chicken feed, using the carotinoid-free 
flock. 
Carotin alone, fed as naturally highly colored butter fat, and the 
orange-yellow pigment of the annatto seed, were found to be without 
influence on the color of the adipose tissue or visible skin parts. Sudan 
III colored only the adipose tissue of non-laying birds, the visible skin 
parts being unaffected by this dye. With laying birds, the egg yolk as 
well as the adipose tissue was colored by Sudan III, but not the visible 
skin parts. 
Yellow corn and green feed only were found to be rich in xanthophyll 
when various plant and animal materials were tested for their xan- 
thophyll content by their effect on the color of the egg yolks when fed 
to hens laying carotinoid-free eggs. A little of the pigment was found 
in hempseed, barley, gluten feed and red corn. Wheat, wheat bran, 
oats, cottonseed meal, rape seed, meat scraps, blood meal, skim-milk 
and butter-milk were found to contain negligible quantities of 
xanthophyll. 
The experiments here reviewed were conducted at the Missouri 
Agricultural Experiment Station with the cooperation of Prof. 
H. L. Kempster of the Department of Poultry Husbandry. The 
complete data appeared in a recent issue of the Journal of Biological 
Chemistry. 
1 Palmer, L. S. and Eckles, C. H., /. Biol. Chem., 17, 1914, (191-249); Missouri Agric. 
Exp. Sta. Bull, Nos. 9, 10, 11, 12, 1914, (313-450). Palmer, L. S., /. Biol. Chem., 23, 
1915, (261-279); 27, 1916, (27-32). 
2 Osborne, T. B. and Mendel, L. B., /. Biol. Chem., 32, 1917, (309-323) ; 34, 1918, (17-27). 
3 Palmer, L. S., Ibid., 27, 1916, (27-32). 
4 Osborne, T. B. and Mendel, L. B., Ibid., 33, 1918, (433-438). 
^ Palmer, L. S. and Thrun, W. E., /. Ind. Eng. Chem., 8, 1916, (614r-618). 
6 Barbieri, N. A., Paris, C. R. Acad. Sci., 154, 1912, (1726-1730). 
7 Blakeslee, A. F. and Warner, D. E., Science, 41, 1915, (432-434); Amer. Nat., 49, 1915, 
(360-368). 
8 Blakeslee, A. F., Harris, J. A., Warner, D. E. and Kirkpatrick, W. F., Storrs Agric. 
Exp. Sia. Bull. No. 92, 1917, (95-194). 
9 Harris, J. A., .Blakeslee, A. F. and Warner, D. E., These Proceedings, 3, 1917, (237). 
10 Warner, D. E. and Edmond, H. D., /. Biol. Chem., 31, 1917, (281-294). 
11 Riddle, O. and Harris, J. A., Ibid., 34, 1918, (161-174). 
12 Barrows, H. R., Maine Agric. Exp. Sta. Bull., No. 232, 1914, (237-252). 
13 Blakeslee, A. F., Storrs Agric. Exp. Sta. Bull., No. 92, 1917, (152). 
