Vol,. 8, 1922 
PHYSIOLOGY: B. MASUROVSKY 
39 
controls. Histological examination of the gonads confirmed the presence 
of very abundant lutein tissue and demonstrated the formation of this 
tissue about the egg in unruptured, normal follicles and in atretic follicles. 
Ripe, normal Graafial follicles were invariably absent. A powerful, specific 
stimulus to lutein cell transformation has thus been ejff^ected by this hormone. 
Aside from the fact that it is tolerated only in smaller quantities, fresh 
posterior hypophyseal substance gave none of the above characteristic 
effects upon growth, oestrus and ovulation in animals as compared with 
similar care with litter mate sisters. 
* Aided by grants from the Committee on Scientific Research of the American Medical 
Association. 
A STUDY OF THE EFFECTS OF CUCURBITA PEPO SEEDS ON 
KIDNEY EXCRETION 
By Benjamin Masurovsky 
From PHYSioiyOGicAi. Chemistry Laboratory, CoIvIvEge of Physicians and Surgeons, 
CoivUMBiA University 
Communicated by R. Pearl, February 4, 1922 
1. Purpose and Plan of Experiment. — The object of this experiment was 
to compare and verify the results obtained from a somewhat similar ex- 
periment conducted during March 1920.^ Moreover, a further study of 
the effects of Cucurbita seeds on kidney excretion is thereby promulgated. 
Since the previous experiment does not give sufficient analytical data 
so far as the urine samples are concerned a more elaborate plan was fol- 
lowed by virtue of which not only comparative data may be obtained but 
additional analyses may be available for the elucidation of the problem 
under consideration. 
Three successive dietary periods were arranged for the exj>eriment, 
namely, three days of a preliminary basal dietary period, three days of 
an experimental dietary period, and three days of a final dietary period. 
The entire experiment lasted for nine consecutive days. 
All the foodstuffs consiuned in this experiment were measured. The 
pumpkin seeds (Cucurbita pepo) were of two kinds, roasted and raw, a 
known quantity of each kind was added to each meal during the experi- 
mental dietary period. 
2. Dietary. — Hindhede's "back-to-the-f arm' ' dietary, - slightly modified, 
was used for the basal diet. Composition of this dietary is given in table 1 . 
This diet was used during the preliminary and final periods of this exper- 
iment. The experimental diet consisted of the same basal diet plus 30 
grams of roasted pumpkin seeds for the first day; 35 grams of roasted 
and 30 grams of raw pumpkin seeds mixed for the second day, and 30 
grams of raw seeds for the third day of the experimental dietary period. 
