40 
PHYSIOLOGY: B. MASUROVSKY 
Proc. N. a. S. 
TABLE 1 
Composition of the Basal Dietary^ 
NAME 
QUANTITY 
PROTEIN 
FAT 
CARBOHYDRATES 
CAI.ORIES 
Water 
600 CC. 
Graham 
bread 
300 gms . 
19.7 
4.8 
153.9 
780 
Apples 
600 gms . 
1.8 
3.0 
76.8 
342 
Milk 
800 CO . 
25.6 
30.4 
40.0 
620 
Butter 
60 gms . 
.6 
48.4 
450 
Potatoes 
800 gms . 
15.2 
.8 
160.0 
800 
Total 
62.9 
87.4 
430.7 
2992 
No salt was used. 
3. Period I. — In securing the daily samples of urine the bladder was 
emptied about 7 a. m., April 3rd, and this urine was discarded. All the 
urine from that hour up to and including that passed the next day at 7 
a. m. was saved, thoroughly mixed, preserved with 5 cc. of toluene, a 
sufficient amount to film the bottom and the walls of the container. The 
latter were wide mouth glass jars with ground glass stoppers. Thus, 
each composite sample represented the mixed excretions for twenty- 
four hours. 
As soon as a twenty-four hour sample was secured it was immediately 
analyzed for the determination of volume, specific gravity, temperature, 
titratable acidity (using 0.1 N NaOH) (4, pp. 508-509), NH3 determina- 
tion (Folin's method) (4, p. 523); total nitrogen determination (Kjel- 
dahl method) (4, pp. 512-513) ; creatinine (Folin's colorimetric method) 
(4, pp. 530-531); urea (Marshall's method) (4, p. 522); uric acid (FoHn 
and Schaffer method) (4, p. 536) ; and total solids of a sample were computed 
by means of Long's coefficient (4, p. 512). The remainder of each of the 
samples was then covered with a layer of toluene to preserve it for the 
inorganic analyses. 
table 2a 
Analyses of Composite Samples of Urine from Period I 
DATE 
1921 
Apr. 3 
Apr. 4 
Apr. 5 
VOLUME 
CC. 
1360 
1600 
1710 
TEMP. 
C. 
24 
22 
24 
SP. GR. 
1.019 
1.017 
1.016 
T. S. 
GMS. 
65.77 
66.56 
69.35 
ACIDITY CC. 
0.1 N NaOH 
PER 100 CC. 
39.0 
24.3 
10.8 
TOTAL N 
GMS. 
11.72 
9.85 
10.04 
DATE 
1921 
Apr. 3 
Apr. 4 
Apr. 5 
TABLE 2h 
Nitrogenous Ingredients Found in Samples of Urine, Period I 
AMMONIA CREATININE URIC ACID UREA UNDETERM. N 
.38 
.48 
.31 
1.07 
1.24 
1.28 
.57 
.65 
.73 
20.28 
17.17 
18.54 
1.37 
.78 
.39 
