Vol.. 8, 1922 
PHYSIOLOGY: B. MASUROVSKY 
43 
7. Inorganic Determinations. — For the determination of total sul- 
phates and ethereal sulphates FoHn's methods were employed; total phos- 
phates were determined by the Uranium Acetate method ; for the deter- 
mination of chlorides and chlorin Volhard- Arnold method was employed. 
TABLE 7 
Mineral Ingredients Found in the Urine Samples, Gms, 
SAMPLE NO. 
CHLORIDES 
Cl 
TOTAL PzOs 
TOTAL SO3 
ETHEREAL SO3 
1 
9.05 
5.43 
1.67 
1.57 
.268 
2 
8.66 
5.20 
1.56 
1.60 
.367 
3 
7.42 
4.45 
1.69 
1.64 
.328 
4.6 
6.61 
3.96 
1.71 
1.76 
.332 
5 
6.51 
3.90 
1.87 
1.78 
.346 
6 
7.86 
4.72 
2.08 
1.97 
.426 
7 
6.83 
4.10 
1 .73 
1.70 
.331 
8 
7.77 
4.66 
3.14 
1.83 
.299 
9 
7.28 
4.39 
1.74 
1.83 
.288 
8. Conclusions. — I. The results obtained in the experiment substan- 
tiate the results of the first experiment inasmuch as the volume and total 
solids output during the experimental Period II was less than in the check 
Period III; the hypothetical volume for the check period being 2140 cc. 
while during the experimental Period II the output was 2047 cc; the 
average output of total solids during the check period being 71.96 while 
during the experimental Period II it was 64.14 gms. 
II. There was 28.12% more uric acid eliminated during Period II than 
during the check Period III. 
III. Also 17% more urea was eliminated during the experimental 
period than during the check Period III. 
IV. The striking difference in the intensity of color in the urine samples 
of the experimental period revealed by the indican test is of pathological 
significance. 
V. From the verified results and additional data obtained in this ex- 
periment the role played by Cucurbit a pepo seeds in animal metabolism 
is of a chemico-pharmacognostic value. 
I wish to express my thanks to Prof. W. J. Gies (Columbia) and Dr. 
B. G. Miller, Jr. (Columbia), for helpful suggestions in this work and to 
Dr. J. G. Lipman, Director of N. J. Agr. Exp. Station, for material assis- 
tance with this experiment. 
* For the sake of uniformity in tliis work the mean of only the second and third 
days' results of each period was taken. For it is evident that the first day's excretions 
are only slightly affected by the change of diet. 
1 Masurovsky, B., "Effects of Some Cucurbita Seeds on Animal Metabolism," 
/. Agric. Res., 21, No. 8, 1921 (523). 
2 Lusk, G., Science of Nutrition, p. 340. 
^ Atwater and Bryant, "The Chemical Composition of American Food Materials,'^ 
Bull. 28 (Revised), U.>S. Dept. of Agriculture, 1902. 
^ Hawk, P. B., Practical Physiological Chemistry, 6th edition, 7 pi. (col.), 1920. 
