PATHOLOGY: W. N. BERG 263 
TABLE 1 
Weights of Euglobulin Obtained from 50 cc. Portions of Serum 
SERUM 
NO. 
WITHOUT 
HEAT 
WITH HEAT 
INCREASE 
IN WEIGHT 
grams 
grams 
grams 
48 
0.774 
1.201 
0.427 
96 
0.547 
0.793 
0.246 
1 
0.406 
0.719 
0.313 
1 
0.435 
0.642* 
0.207 
1 
0.243 
0.304 
0.061 
* Low result, due to incomplete flocculation. 
TABLE 2 
Analyses of Heated and Unheated Sera. All Weights Calculated to Grams in 
10 cc. Serum 
ANTHRAX 
TETANUS 
DIPHTHERIA 
48 not 
heated 
48 
heated 
96 not 
heated 
96 
heated 
1 not 
heated 
1 
heated 
1 not 
heated 
1 
heated 
1 not 
heated 
1 
heated 
(a) Total coagulable pro- 
0.618* 
0.618 
0.788 
0.788 
0.558 
0.558 
0.665 
0.665 
0.665 
0.665 
(b) Euglobulin 
0.155 
0.240 
0.109 
0.159 
0.049 
0.061 
0.081 
0.144 
0.087 
0.128t 
(c) Pseudoglobulin and 
albumin estimated to- 
gether 
0.437 
0.378 
0.668 
0.625 
0.447 
0.451 
0.534 
0.487 
0.523 
0.484 
(d) Pseudoglobulin 
0.290 
0.211 
0.568 
0.535 
0.385 
0.378 
0.475 
0.397 
0.479 
0.438 
(e) Albumin 
0.191 
0.191 
0.133 
0.135 
0.103 
0.103 
0.090 
0.098 
0.087 
0.097 
Pseudoglobulin converted 
into euglobulin 
27% 
6% 
0.2% 
16% 
9% 
* The same figure for total coagulable protein applies to both the heated and not heated 
serum; these are not exact duplicates. 
tLow result; see table 1. 
lively large volumes of the precipitates, solubility of precipitate in the 
washwater, occluded ammonium sulphate, etc. All the errors however, 
do not invalidate a comparison of the data in one column with those on 
the same serum, in the next column. 
The figures for albumin are practically the same in the heated and 
unheated sera. The figures for pseudoglobulin are consistently lower in 
the heated sera than in the unheated; this is an almost necessary con- 
sequence of the transformation of part of the pseudoglobulin into some 
protein having several of the precipitation characteristics of euglobulin. 
What is most important for the present investigation is that the loss 
of pseudoglobulin in the heated sera corresponds almost quantitatively with 
the gain in euglobulin in the same sera. 
To calculate the percent of pseudoglobulin transformed into eu- 
globuhn by the heat treatment; the difference between the amount of 
