366 
AGRICULTURE: JOHNS AND JONES 
tained by dialysis or by saturating the filtrate with ammonium sulfate. 
This second globulin we have named conarachin. 
Isolation of Arachin. — An extract was made by mixing 500 grams of 
peanut meal with 2.5 liters of a 10% aqueous solution of sodium chlo- 
ride. This mixture was then run through a mill and the resulting thin 
liquid was filtered clear through paper pulp. To the filtrate, solid am- 
monium sulphate was added gradually until the solution became 0.2 
saturated with this salt. A precipitate began to appear at 0.15 of satu- 
ration and increased rapidly until 0.2 of saturation was reached, when 
precipitation practically ceased and did not occur again until much 
more ammonium sulfate was added. On allowing the 0.2 saturated so- 
lution to stand overnight, the precipitate settled in a very compact 
form so that the supernatant Hquid could be easily decanted. The pre- 
cipitate was washed with a 10% sodium chloride solution containing 
ammonium sulphate to 0.2 of saturation. The residue was then redis- 
solved in a small volume of 10% sodium chloride, and the resulting 
solution was filtered and dialyzed until chlorides were removed. In 
this manner the greater part of the globuHn present in the meal was 
obtained as a white powder. This was suspended in alcohol for twenty- 
four hours and in anhydrous ether for another twenty-four hours. It 
was then filtered off and freed from ether in a vacuum desiccator and 
finally dried to constant weight at 110°C. 
Isolation of Conarachin. — The filtrate from which arachin had been 
removed, and which was now 0.2 saturated with ammonium sulphate, 
was completely saturated by adding more ammonium sulphate. This 
treatment produced a small quantity of precipitate. This was redis- 
solved in 10% sodium chloride and the filtered solution dialyzed until 
free from chlorides. The conarachin was then dried in the same man- 
ner as the arachin. 
Isolation of an Albumin. — Beside arachin and conarachin sodium 
chloride solutions also extract from peanut meal a trace of albumin 
which coagulates at 65 to 70°C. Carbon and hydrogen determinations 
made on a small quantity of this albumin gave results which agree 
closely with the values obtained by Osborne^ and his co-workers for the 
legumeHns frequently found in the seeds of leguminous plants. 
The difference in the composition of arachin and conarachin is shown 
by the following analytical results: 
ARACHIN CONARACHIN 
Per cent Per cent 
C 52.15 51.17 
H 6.93 6.87 
N 18.29 18.29 
S 0.40 1.09 
0 22.23 22.58 
