514 
PHYSIOLOGY: /. LOEB 
The limiting lower concentration to bring about the recovery of 
70% or more eggs in twenty-four hours depends in a definite way 
upon the nature of the anion of all these salts, being for CI: SO4: citrate 
in the ratio of approximately 1:4: 16, as stated in a previous publication.^ 
In time recovery took also place in the solutions below the limiting 
concentration. In the recovery experiments no antagonistic salt action 
in noticeable, probably on account of the fact that the decisive boundary 
is the inner surface of the membrane which receives only traces of the 
salt from the outside solution, enough to accelerate the diffusion of KCl 
but not enough to inhibit this process. 
Table II shows the behavior of the eggs in twenty-four hours when put 
into solutions of non-electrolytes. The characteristic influence of 
increasing concentrations of the solution upon recovery which is so 
striking in Table I is entirely lacking in this case, m/2 solutions of non- 
electrolytes acting no better than weaker ones. What little recovery 
takes place in the solutions of non-electrolytes is 'accidental' and where 
the figures for recovery are 20% or more, it is probably due to the second- 
ar}^ formation of some acid or alkali through microorganisms or in the 
embryo. The main fact is that the number of hearts which recovered 
did not increase when the eggs were left for a number of days in the 
solution of non-electrolytes. When the eggs were transferred from the 
solution of non-electrolytes to a solution of the proper electrolyte the 
*salt effect' and hence the recovery took place. 
TABLE II 
PERCENTAGE OF HEARTS WHICH RECOVERED FROM KCl POISONING IN 
00 
"* 
cs 
10 
0 
> 
CM 
g 
C 
£ 
e 
a" 
cs 
\ 
s 
1- 
0 
K 
Glucose 
0 
15 
10 
5 
0 
0 
0 
5 
0 
0 
0 
15 
Cane sugar 
14 
10 
14 
20 
14 
15 
15 
37 
21 
32 
10 
5 
Glycerin 
20 
20 
15 
11 
11 
0 
15 
5 
15 
10 
Methyl alcohol 
20 
45 
25 
15 
20 
10 
10 
10 
20 
10 
15 
10 
Ethyl alcohol 
5 
25 
25 
35 
0 
15 
5 
5 
15 
10 
Urea 
5 
40 
15 
10 
5 
30 
10 
25 
10 
20 
15 
10 
Table III illustrates the effect of the cation suggesting an influence 
of the periodic law. Thus recovery occurs neither in RbCl and CsCl 
nor in the salts of the alkali earth metals occupying the corresponding 
position in the next group of the periodic series; namely, Sr and Ba. 
These cations act by preventing the diffusion of the KCl from the egg 
for if we transfer the eggs from such a solution to one with an efficient 
