Archer — On Freshwater Rhizopoda. 
79 
exterior, or thickened and consolidated, and altered ectosarc to the 
body-mass, I think this experiment would demonstrate the point. If 
the rhizopodand its investment were like endosarc" and " ectosarc," 
I should suppose that this experiment must also have given, in this 
regard, a similar result to the preceding. But this experiment, the 
effect of which in a single specimen I depict in Fig. 5, gave other 
curious results. As I have already described, the body-mass pre- 
sented a stratum of the pale, shiny, elliptic bodies, just under its peri- 
phery, and immediately beneath this, in the majority of the examples, 
they presented also the more or less dense stratum of bright chlorophyll- 
granules ; and within all, generally at the side most remote from the 
pseudopodium-bearing region, they admitted of being seen (with pa- 
tience) the elliptic nucleus." JN^ow the immediate effect of the present 
re-agent was, as it seems to me, highly curious and interesting. I 
have said the sarcode mass coagulated into one or several balls, leaving 
the mantle bare, but not only did it do so, but these balls, in con- 
tracting, carried with them and huddled together the elliptic shiny 
bodies, which in the normal state formed the outer stratum, or that 
the more distant from the centre ; whilst, at the same time, the chlo- 
rophyll-granules were left outside the contracted sarcode balls, though 
they, in the normal state, formed the inner stratum, or that nearer the 
centre. Thus, a complete transposition taking place in a moment, that 
which had been the outer being carried in, and those which had been 
the inner left out. Further, in the majority of the instances in which 
this experiment was tried, the nucleus was likewise not included by 
any of the sarcode balls, but left outside as a somewhat shrivelled or 
lobed pale greyish-bluish coloured, rather shiny, body ; in other in- 
stances, however, I could not again find the nucleus, and it must have 
either been embedded in some of the balls of sarcode or ejected, and 
got lost. The action of the present reagent on the mantle or coat 
itself, seems to be that of causing its expansion or inflation, as it as- 
sumed a nearly circular and somewhat enlarged outline ; the specimens 
which happened to be experimented upon, were some in which the ex- 
ternal hair-like processes were very short, yet quite distinctly marked, 
nor did the action of the re-agent cause any very great alteration in 
their aspect, whilst the general surface retained the colourless charac- 
ter and the dotted appearance due to the linear markings in the sub- 
stance of the coat, or to the hair-like processes themselves ; whilst at 
the periphery, just as in the normally empty coat, where a thicker 
mass of the substance is seen rim-like, and where, of course, we thus 
look through a greater density, it appears of a bluish colour. Upon 
adding a very little of the ordinary tincture of iodine, the coat took 
a straw colour, the other portions remaining as before. This experiment, 
therefore, was not without very instructive results. 
The action of sulphuric acid was also interesting. Brought to 
bear very slowly at first, this time upon examples showing no chloro- 
phyll-granules, this re-agent caused a slight inflation or expansion of 
the total rhizopod, coat and all, simultaneously. One specimen, pre- 
