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became increasingly feebler, the reflex had almost disappeared after 3^/3 hours. 
At the trunk, but especially at the extremities, slight symptoms of stimulation 
occurred, which manifested themselves in faint inco-ordinated movements. Once 
there occured even opisthotonos, lasting a few seconds. After 5 hours no reac- 
tion took place, and after 18 hours it was found that rigor mortis had set in. 
Section: The stomach, which was in a highly hyperaemic condition, con- 
tained blood-stained mucus; the mesentery showed slight, the mucosa of the 
cavity of the mouth and of the gastro-intestinal canal but very little hyperaemia; 
the lectum alone was more strongly injected. The nerves and muscles reacted 
normally on electric stimulation. 
Experiment No. 6. A frog weighing 50 g, which was in a somewhat 
enfeebled condition owing to previous experiments, received subcutaneously 0,1 g 
sodium anthranilate. After one hour the animal was already greatly exhausted. 
Death occured after 4 hours. 
Section: The mesentery was not hyperaemic. The alimentary canal, with 
the exception of the mucus membrane of the mouth, was slightly injected. The 
stomach contained blood-stained mucus. 
Experiment No. 7. A medium-sized frog was given 0,1 g sodium anthran- 
ilate. No symptoms w^hatever occurred, not even the slightest. 
Experiment No. 8. A frog weighing 58 g was injected on each of two 
consecutive days with 0,15 g sodium anthranilate , and on the third day 
with 0,2 g. The injections were also tolerated without consequent symptoms. 
Experiments No. 9 and 10. One frog of 68 g received 0,2 g, and another 
of 60 g 0,15 g sodium anthranilate. No consequent symptoms whatever 
occurred. 
Experiment No. 11. A frog weighing 69 g was given 0,4 g sodium anthran- 
ilate. After 4 hours isolated inco-ordinated movements were still observed; 
the reflex actions had completely disappeared, the action of the heart had 
become considerably slower. After 4^^ hours 0,000075 g nitrate of strychnine 
was injected. After 6 hours (i. e. 1^4 hours after the strychnine injection) 
convulsions and slight spasms of the extremities, of short duration, occurred on 
stimulation. No strychnine-action could be observed on the musculature of the 
trunk. After 18 hours the animal was found dead. 
It follows from the foregoing that anthranilic acid para- 
lyses the central nervous system of the frog. The tonic 
spasms (which, however, were only slight) observed before 
the occurrence of absolute paralysis, are no doubt due to 
a less intense and transitory stimulation of the muscles or 
peripheral nerves. The ataxic movements, however, are 
probably due to the comparatively early paralysis of centri- 
petal ducts, and the loss of controlling sensations thereby 
caused, with comparatively well-preserved motor-power. 
Strychnine in large doses, although it allows again a slight 
flicker of the reflex-action which had already disappeared, 
is not capable of completely removing the paralysing effect 
of anthranilic acid, or to prevent death. The fatal dose 
amounts to about 3,0 g per kilogram weight of the frog. 
The urine of 24 hours of several frogs which had received an injection of 
anthranilic acid, was collected. After addition of potassium - bismuth iodide, 
a strong precipitate of a yellow-red colour was formed. The urine was now 
filtered, the residue on the filter collected, and decomposed with potash liquor; 
8 
