In spite of the most careful examination (compare experiment No. 13) the acid 
obtained in the first experiment on the dog, could not now be produced. 
Experiment No. 18. I personally took per os 2,0 g sodium anthranilate, 
viz., twice 1,0 g with an interval of 2 hours. Half an hour after each ad- 
ministration a slight attack of perspiration occurred without increase of tem- 
perature. About 3 hours after the last dose a slight but yet troublesome 
salivation was noticeable. Apart from a slight feeling of discomfort in the 
abdomen, which occurred 12 to 15 hours after administration, disturbances on 
the part of the gastro-intestinal canal remained absent. The stools were 
normal. The urine, collected in 48 hours, had an acid reaction; it showed an 
extremely intense fluorescence, contained no albumin, and reduced copper 
solution. The fermentation- test had negative, and the phenylglucosazone-test 
positive results. When soda was added, the fluorescence disappeared. No 
attempt was made to isolate anthranilic acid. No hippuric, benzoic, or any 
other organic acids whatever could be obtained from the urine which was treated 
in the same manner as described in experiment No. 13. 
These experiments show that warm-blooded animals 
tolerate without lasting injury the injection, or administra- 
tion per OS, of anthranilic acid in considerable doses. In 
the worst case, diarrhoea and salivation occur. The sub- 
stance is found back in the urine in an unchanged con- 
dition, and can already be recognized without chemical 
examination by the fluorescence. In addition to the sub- 
stance, sugar is found in the urine of man and dog; in that 
of the rabbit, however, sugar is either not found at all, or, 
if very large doses have been given, only traces are present. 
The sugar can be detected either forthwith by means of 
the phenylglucosazone-test, or less readily by the fermen- 
tation-test, as our substance, like many other reducing 
bodies, impairs the fermenting power of the yeast. 
It was not possible to detect any action of anthranilic acid on 
bacteria. 
One cc of a 3 per cent, solution of sodium anthranilate was added 
to cultures of each Bacteriiun coli, Staphylococais alb us and Bacillus 
subtilis, which had been inoculated in about 4 cc peptone-w^ater. When 
the substance had been in contact with the bacteria for 48 hours 
at a constant temperature of 34°, the cultures were inoculated into 
alkaline peptone-agar. Their growth was vigorous and showed no 
difference from that of control - cultures which had not been treated 
with anthranilic acid. It follows that sodium anthranilate, in 
a 0,06 per cent, solution, during 48 hours' action, does not 
exert any demonstrable influence on Bacterium coli. 
Staphylococcus albus, and Bacillus subtilis. 
It was also impossible to prove any influence of the 
sodium salt of anthranilic acid on haemachrome and on the 
red blood corpuscles. 
