— 122 — 
precipitate. This reaction is so delicate that even in 0,00000000000 r 
= — g of the sulphate, methyl ester of methyl anthranilic acid could 
be detected. 
When mercuric iodide is added to a sulphate solution, no precip- 
itate is formed. But after prolonged standing bright-yellow prisms 
crystallise out. Of some few of these prisms the angles are truncated. 
With potassium -bismuth iodide the sulphate forms a red precip- 
itate, from which in a few days magnificent red prisms separate out. 
In an acid and highly concentrated solution of the salt, a white, 
delicately violet -coloured precipitate is formed when phosphotungstic 
acid is added. 
If to a sulphate solution a concentrated solution of iodine and 
potassium iodide, or the so-called Flore nee- solution, be added, a thick, 
brown precipitate is formed ; this precipitate, after several hours, crystal- 
lises out in the form of brown, sharp needles, which arrange themselves 
in the form of stars or in druse-form. These also form a blue precip- 
itate with red prussiate of potassium and ferric chloride. 
The physiological effect of methyl ester of methyl anthranilic acid 
in the animal organism is unimportant, like that of the other sub- 
stances already discussed. 
For the following experiments, an aqueous and exactly neutralised 
solution of the sulphate was always selected. 
Experiment No. i. A large strong frog received a subcutaneous injection 
of 0,05 g of the salt. The animal gradually became more and more feeble; 
after hours, it made at first only faint and fruitless efforts to change 
from the dorsal to the abdominal position, but subsequently, when in the 
dorsal position, it made powerful swimming movements with the hind legs. 
The reflex-action was scarcely diminished; respiration and action of the heart 
were not impaired. After 4 hours the first symptoms of recovery became 
apparent. 
Experiment No. 2. The same frog, after 24 hours, when it had com- 
pletely recovered, received 0,075 g- The same action was observed. 
Experiment No. 3. A frog weighing 52 g received 0,07 g. After an 
hour the animal was already very weak. The reflex -action of the anterior 
extremities ceased after 2 hours, that of the hind legs had slightly diminished. 
The action of the heart remained constantly good. After 20 hours the animal 
had recovered. 
Experiment No. 4. A frog weighing 65 g received 0,075 g- After one 
hour feebleness occurred which increased up to the third hour. At this time 
slight disturbances of the co-ordination were also observed. For the rest, the 
symptoms and the course were the same as in experiment No. I. 
Experiment No. 5. The same frog, two hours after an injection of 0,1 g, 
was very exhausted; the reflex- action diminished more and more, was only 
very feeble after 2Y2 hours, and had completely ceased after 16 hours. Not 
until after 43 hours some slight reflex-action of the extremities could again 
be excited by stimulation. The action of the heart had throughout this time 
remained good and strong. After 54 hours the animal had recovered. 
