— — 
Experiment No. 6. A frog weighing 26 g received 0,15 g of the sub- 
stance. After 10 minutes the animal had already ceased to react, and the 
action of the heart could no longer be observed. After 9 hours rigor mortis 
had set in. 
Section: The skin was normal, the cavity of the mouth slightly red; the 
stomach, which contained a glutinous, glassy mucus, was strongly injected. 
The oilier organs were in no way abnormal. The nerves and muscles responded 
normally to electric stimulation. 
Experiment No. 7. A frog weighing 45 g received a dose of 0,2 g. The 
animal, which ceased to react aheady after a few minutes, was injected after 
15 minutes with 0,00005 g strychnine nitrate. This only caused convulsive 
movements, but no tetanus. The action of the heart still continued for a 
comparatively long time, although it was scarcely perceptible. After 20 hours 
the animal was found dead and rigid. 
The section gave the same results as in experiment No. 6. 
Methyl ester of methyl anthranilic acid administered to 
frogs paralyses the central nervous system, and its toxic 
action is not appreciably influenced by strychnine. The 
fatal dose of the sulphate of the ester is about 4,0 g per 
kilogram weight of the frog. 
Experiments Nos. 8 and 9. Doses of 0,5 g and 1,4 g of sulphate of 
methyl ester of methyl anthranilic acid (subcutaneously) had no perceptible 
effect to rabbits. 
Experiment No. 10. A medium-sized dog also tolerated 2,1 g very well. 
It should be mentioned, however, that both in the dog and in the rabbit, 
during the 24 hours after injection, slight catarrhal symptoms of the superior 
respiratory passages occurred. 
The urine of both the rabbit and the dog contained neither albumin 
nor sugar. 
The urine of the rabbit, which possessed the aromatic odour of methyl 
ester of methyl anthranilic acid, after having been acidified with sulphuric 
acid, was reduced to about one fourth its volume, then rendered decidedly 
allcaline by soda liquor, and extracted with ether. The ether showed an 
intense blue fluorescence. After adding a few cc water, the ether was slowly 
driven off. There remained on the water numerous small drops of oil 
coloured brown-black by dirty matter. Attempts to remove the adhering dirt 
from the oil drops did not succeed. With potassium ferricyanide and ferric 
chloride the drops formed a strong blue precipitate. As soon as a few drops 
concentrated sulphuric acid were added to the water, the oil dissolved in the 
latter. In the presence of Florence- solution a brown precipitate was formed, 
which changed into crystals of druse-like formation. A solution of potassium 
permanganate was quickly discoloured, without the occurrence of the previously 
described hues. 
The aromatic odour of methyl ester of methyl anthranilic acid in the 
urine of rabbits; the fluorescence of the ether; the intense Berlin-blue reaction 
of the oil drops; the precipitate and the crystals from the Florence- solution; 
the difficult solubility of the oil in the presence of acids; all these facts prove 
that it can only have been a question of methyl ester of methyl anthranilic 
acid. The fact that potassium permanganate was discoloured without the 
occurrence of those hues, was possibly due to contamination of the ester. 
From the urine of the dog the oil-drops could also be obtained in the 
same manner. They showed the same behaviour. 
