— 124 — 
Finally, in the urine of the frogs the presence of the ester could be 
demonstrated by means of iron. The urine was strongly shaken with ether. 
After driving off the latter, and treating the scarcely visible fatty residue 
with potassium ferricyanide and ferric chloride, the blue colouring matter was 
precipitated. 
It follows from the foregoing that methyl ester of methyl 
anthranilic acid is tolerated by warm-blooded animals in 
comparatively large doses without injurious effect, and 
without the occurrence of any apparent disturbances in the 
organism whatever. 
The substance therefore again passes out of the animal 
organism as such. But the ester is separated out not only 
by the kidneys, but also by the superior respiratory pas- 
sages. This conclusion at least is justified by the catarrhal 
symptoms occurring in the dog and the rabbit after the 
injections. 
In spite of its low physiological action, methyl ester of methyl 
anthranilic acid possesses the properties of a bactericide. 
The experiments with this substance made for the purpose of 
determining the intensity of the action on bacteria, were carried out 
in this manner, that to a measured quantity of peptone-water into 
which the bacteria had been inoculated, 24 hours after the inoculation 
was added one cc of an aqueous solution containing a definite per- 
centage of the sulphate of methyl ester of methyl anthranilic acid. 
When the ester had acted for 24 hours, the micro-organisms 
were again inoculated into alkaline peptone -agar. The temperature 
of the incubator in which the bacteria were placed, was kept throughout 
at 34°. 
The aqueous solutions of the ester wxre freshly prepared for each 
experiment, and not only acid solutions were employed, but also such 
which had been exactly neutralised by soda. This was done not so 
much with the view of removing the small quantity of free sulphuric 
acid (which, as a matter of fact, could not exert any influence on the 
bacteria selected), as for this reason, that possibly the action of pure 
methyl ester of methyl anthranilic acid might differ from that of the 
ester combined with sulphuric acid. 
The experiments were made at first with Bacterium coli, Staphylo- 
coccus albus and Bacillus subtilis. But as the development of the last- 
named was not retarded even in a 0,15 per cent, solution of the 
ester, only the two first-named micro-organisms were employed in the 
subsequent experiments. The results, which were frequently checked, 
are given in the following tables. 
