126 
These two tables do not show any pronounced difference 
in the results. The bactericidal action of methyl ester of 
methyl anthranilic acid within 24 hours asserts itself in the 
case of Bacterium colt already in a 0,05 per cent., and in that 
of Staphylococcus albus in a 0,06 per cent, solution of the 
sulphate, whilst the action of the solutions which have been 
neutralised with soda is less powerful. This difference in 
the action of the two solutions is not very great in the case 
of B acterium coli, but yet noticeable; in that of Staphylo- 
coccus albus, however, it is very marked. In order to destroy 
this micro-organism in 24 hours, a 0,06 per cent, acid solu- 
tion, or a neutralised solution of 0,15 per cent, is required. 
It would appear that the free sulphuric acid, which in such 
dilution as in these experiments does not impair or weaken , 
but rather favours the development both of Bacterium coli 
and of Staphylococcus albus, in the presence of the ester still 
enhances the bactericidal effect of the latter. 
Hsemachrome and red blood corpuscles are not attacked 
either by methyl ester of methyl anthranilic acid, or by a 
neutralised solution of its sulphate. But an acid sulphate 
solution immediately causes the formation of methsemoglobin, 
with lixiviation of the red blood corpuscles, an action which 
of course does not result from the presence of the ester, but 
from that of the sulphuric acid. 
Acetyl compound of methyl ester of methyl anthranilic acid. 
The acetyl compound of methyl ester of methyl anthranilic acid 
is a yellow oily substance, which has a bitter and slightly pungent 
taste, and which solidifies at 15^. In the undiluted state this body 
has a faint, offensive odour, and only when it is strongly diluted it 
acquires a penetrating aromatic sweetish odour which distantly re- 
minds of the odour of orange -flowers. In alcohol, ether, and water 
the oil is soluble in every proportion. The substance shows no 
fluorescence. 
It does not reduce solutions of copper, nor of gold, platinum, 
silver, potassium chromate, and potassium permanganate. 
When Florence -solution is added to an aqueous solution of this 
substance, a brown precipitate is formed, which after several hours 
crystallises out in the form of hexagonal light-brown laminae and 
