— 128 — 
Section: The abdominal wall, stomach and intestine were strongly injected. 
The injected condition of the intestine diminished towards the cloaca. 
A portion of the skin on which the papillae were situated, after being 
hardened, w^as placed in paraffin, and stained. When examined under the 
microscope, it was found that at the places where the papillae were situated, 
the layer of epithelium was raised. The contents of these vesicles consisted 
of a few detached epithelial cells which were partly already whithout nucleus, 
and a small quantity of detritus. At a few isolated parts of the skin which 
under the microscope did not externally show any change, the epithelium was 
also already raised. 
Experiment No. 4. A middle-sized frog received the same dose. After 
one hour the animal was absolutely without reaction. Death occurred after 
about 36 hours. 
The section showed the same results as in experiment No. 3, except that 
the abdominal wall was not hypersemic. 
Experiment No. 5. A medium -sized frog received 0,1 g. After ^^^^ 
the animal had lost all power of reaction; the action of the heart had dimin- 
ished and become slower. After 24 hours the frog was found dead. 
Section: The intestine showed general hyperaemia especially well-marked 
in certain circumscribed spots. The stomach which was also in a strongly 
injected condition, contained blood-stained mucus. The skin showed no 
peculiarities. 
When the undiluted substance was injected, it acted already after a few 
minutes. 
Experiment No. 6. A frog weighing 25 g received an injection of 0,14 g 
of the methyl ester of acetyl methyl anthranilic acid which I had received 
with the second consignment. After 10 minutes the animal had ceased to react. 
On electric stimulation the muscles and nerves reacted in a normal manner. 
The action of the heart was slow and feeble. After 15 minutes an injection 
was made of 0,00005 g strychnine nitrate; this produced no convulsions, but 
only slight twitching in response to stimulation. After 20 hours the frog was 
found rigid. 
Section: The skin was normal, the cavity of the mouth shghtly red; the 
stomach and intestine were strongly hypersemic. The latter contained blood- 
stained mucus in addition to faecal matter. 
It must appear remarkable that in other frogs the formation of 
vesicles on the skin did not occur, either when smaller doses were 
given than in experiment No. 3 , or in the case of equal or larger 
doses; and that, in fact, the abdominal wall of those animals was not 
even hyperaemic. At first the view was not wholly unwarranted, that 
possibly, in consequence of secretion of the substance through the skin, 
irritation of the latter by the essential oil might have occurred, followed 
by the formation of vesicles. This view, however, had to be abandoned, 
when it was subsequently found that the acetyl-group is split off in the 
body of the frog, and only the methyl ester of methyl anthranilic 
acid is excreted. But the same action on the skin was not observed 
in the case of methyl ester of methyl anthranilic acid (compare page 122). 
Moreover, I do not believe that the acetyl compound alone is 
capable of producing such comparatively powerful changes, without 
acting in an equally strong manner in the interior of the organism. 
