— 129 — 
especially on the intestine and stomach. The occurrence of the 
vesicles on the side of the abdomen rather points to some external 
local cause. 
The frog survived by 3Y2 days the one (experiment No. 4) which 
had received the same dose of 0,09 g, and by 4 days the other one 
(experiment No. 5) which had received 0,1 g. The action, therefore, 
was considerably less powerful. It is probable that shortly after the 
injection a few drops of the acetyl compound had again flown out 
of the puncture canal, and as the plate on which the frog was placed, 
was not kept very damp, those drops could exert a caustic action on 
the abdomen of the animal. 
The acetyl compound of methyl ester of methyl anthran- 
ilic acid, like the other substances already discussed, para- 
lyses the central nervous system of the frog, but this toxic 
effect occurs comparatively rapidly, in any case at a con- 
siderably earlier period than with the other substances. 
Strychnine administered after poisoning with the acetyl 
compound, also induces a slight revival of the extinguished 
reflex-actions, but for the rest does not possess any anti- 
dotal properties. The fatal dose of the acetyl compound of 
methyl ester of methyl anthranilic acid amounts to 1,5 g 
per kilogram weight of the frog. 
Before discussing the examination of the urine of the frogs, I will 
deal with the experiments made with this acetyl compound on warm- 
blooded animals. 
Experiment No. 7. A guinea pig, weighing 500 g, received 0,55 g sub- 
cutaneously. After a few minutes it made some ataxic movements, fell down 
on its side, and fell asleep. The reflexes of the hind legs were much reduced, 
and had completely disappeared after 3 hours; those of the anterior extremities 
still remained indicated for a long time. Occasionally the animal, in the dorsal 
position, spontaneously made some slow walking - movements with the front 
legs ; at the hind legs there occurred only short single twitches at intervals of 
from I to 15 minutes. The respiration gradually became slower and very 
superficial ; the frequency of the pulsations of the heart also became reduced, 
but the energy of the contractions, as far as it could be judged after the punc- 
ture was made, still remained normal. After hours the corneal-reflex also 
ceased. During somnolence about 2 cc alkaline, milky urine could be pressed 
out of the bladder. After 19 hours the animal was found dead and rigid. 
Section: At the injection- spot a briny oedema had occurred. Hypersemia 
could not be detected in any of the organs. The intestine was moderately 
filled with formed fsecal matter. Kidneys, liver, lung and heart showed no 
peculiarities. 
Experiment No. 8. A small rabbit received subcutaneously 0,5 cc of the 
undiluted acetyl compound of methyl ester of methyl anthranilic acid. After 
a few minutes it made inco - ordinate movements, fell down, and fell asleep. 
The power of reaction of the hind legs was much reduced. Respiration and 
pulsation were neither slower nor more feeble. After lying down for half an 
9 
