— 131 — 
the oil-drop carefully cleaned, and then placed in a solution of potassium ferri- 
cyanide and ferric chloride. The solution assumed an intense blue colour. The 
other oil -drops, after treatment with Florence- solution, produced beautiful 
fascicular crystals. 
The urine of the dogs contained neither albumin nor sugar. The ethereal 
extract of the urine which had first been acidified with sulphuric acid, and 
after concentrating had been rendered alkaline with soda liquor, also showed 
a blue fluorescence, but only slightly. After driving off the ether, only a few 
small oil-drops floated on the water which had been added, and these formed 
a strong blue precipitate with iron. 
Experiment No. 13. A medium-sized rabbit received a subcutaneous in- 
jection of 1,0 g of a solution of methyl ester of acetyl methyl anthranilic acid 
from the second consignment. After half an hour the animal became sleepy, 
and extended the fore legs. When placed in the dorsal position it turned 
over with difficulty. Pulsation, respiration and reflex-action remained normal. 
After 4 hours the animal had to some extent recovered. 
The urine secreted in 48 hours had an alkaline reaction, contained no 
albumin, reduced copper solution, and gave a positive phenylglucosazone- 
test. By means of the fermentation-test it was found to contain 1,1 per cent, 
sugar. The urine Avas now acidified with sulphuric acid, slowly evaporated 
almost to dryness, again rendered alkaline with soda, and extracted with 
alcohol. After driving off the alcohol, the residue was extracted with ether. 
The latter showed an intense blue fluorescence. When the ether, after adding 
a small quantity of water, had again been driven off, there floated on the 
water numerous small and most minute drops of oil which showed all the 
reactions of methyl ester of methyl anthranilic acid. 
Experiment No. 14. A medium-sized dog received a subcutaneous in- 
jection of 3,0 g of a solution of the substance received with the second con- 
signment. This dose did not give rise to any symptoms. The urine of 
48 hours measured only 30 cc. It had an alkaline reaction, and contained 
neither albumin nor sugar. The urine was treated in the same manner as in 
the last experiment. Fluorescence of the ether could not be observed with 
certainty. After driving off the ether, no oil-drops floated on the added water. 
Although this liquid produced an intense blue precipitate with potassium ferri- 
cyanide and ferric chloride, the reduction of metal salts was only slight. 
Finally, the urine of the frogs which had received an injection of the 
acetyl compound, formed with Florence- solution a precipitate, from which, 
however, no crystalline needles were precipitated when soda and hydrochloric 
acid were added, a proof that the acetyl compound was not present in 
the urine. 
As the presence of methyl ester of methyl anthranilic acid was suspected, 
the small residue of an ethereal extract of the carefully evaporated urine was 
treated with potassium ferricyanide and ferric chloride. A strong blue preci- 
pitate confirmed the supposition. 
As is shown by the experiments on warm-blooded 
animals, the acetyl compound of methyl ester of methyl 
anthranilic acid appears to be fairly indifferent for the 
organism of the dog, as doses of even 3,0 g were tolerated by 
a medium-sized animal without the slightest demonstrable 
injuries to the organs, and without general disturbances. 
Nor was the appetite reduced. No sugar was excreted by 
the organism. 
9* 
