— 132 — 
With the guinea pig and the rabbit the conditions are 
different. After the introduction of moderate doses, the 
substance causes in these animals at first slight disturbances 
of the co-ordination, but soon afterwards they become stupe- 
fied. The narcotic effect is, however, not prolonged. After 
stronger doses the central nervous system becomes para- 
lysed. For guinea pigs the fatal dose is i,o g per kilogram 
weight. Apart from these nervous symptoms, the substance 
produces in the rabbit a considerable excretion of sugar, 
which can be readily detected by the fermentation- and 
phenylglucosazone-tests. 
The acetyl compound of methyl ester of methyl anthran- 
ilic acid is not stable in the organism of frogs, guinea pigs, 
rabbits and dogs, but is excreted as methyl ester of methyl 
anthranilic acid after the acetyl-group has been split off. 
On micro-organisms the acetyl compound of methyl 
ester of methyl anthranilic acid has no influence. The 
development of B acterium coli. Staphylococcus alb us, and 
Bacillus subtilis — the cultures had been inoculated into 
about 4 cc peptone- water — was in no way impaired by the 
addition of i cc of a 3 per cent, solution acting for 3 days. 
The question now presented itself, whether per contra the bacteria 
had exerted any action on the substance. Such action, in view of 
the composition of the substance, and of the results of the experi- 
ments on animals, could, naturally, only have consisted of the splitting 
off of the acetyl group. 
When the bacteria had been in contact for 4 days with the 
acetyl compound of methyl ester of methyl anthranilic acid, the cul- 
tures were submitted to the following examination. 
Culture of Bacterium coli. 
1. To one part peptone-water were added, after acidification with one 
drop dilute sulphuric acid, a few drops of a solution of potassium perman- 
ganate. Although it was at once discoloured, the various hues observed with 
the solution of the pure sulphate of methyl ester of methyl anthranilic acid did not 
occur. Nor could a dirty-blue fluorescence of the peptone-water be recognised. 
2. A small quantity of Fl or en ce- solution was added to one part peptone- 
water. Although a stronger precipitate was formed, no crystallisation took 
place even after several week's standing. 
3. Another part was slowly evaporated, extracted with ether, and the 
ether driven off. The residue, treated with potassium ferricyanide and ferric 
chloride, formed only a very slight blue precipitate. 
As a check, about 4 cc peptone-water which had also been charged with 
Bacterium coti, but to which no acetyl compound had been added, was eva- 
porated to dryness and extracted with ether. The residue in this case also 
formed only a slight blue precipitate. 
