— 133 — 
Culture of Staphylococcus albus. 
The examination was conducted in the same manner as with Bacterium 
coh\ and led to the same results. 
Culture of Bacillus subtilis. 
1. A portion of the peptone-water, after acidification, discoloured a solution 
of potassium permanganate. The change of colours was here also absent, 
but a dirty -blue fluorescence could be observed, which, however, was but 
very faint. 
2. The precipitate formed after adding Florence- solution, did not crys- 
tallise out. 
3. The residue from the ethereal extract of peptone- water, when treated 
with iron, formed a strong blue precipitate. But an equally strong precipitate 
was obtained from a pure peptone-water culture of Bacillus suhtiLis. 
It follows that B acterium coli and Staphyloc occiis albus 
have no influence on the acetyl compound of methyl ester 
of methyl anthranilic acid. Such a result was to be expected 
in the case of these two micro-organisms, for if the acetyl- 
group had split off from only one-twelfth part of the sub- 
stance, the micro-organisms would have been destroyed. 
The slight blue reaction at any rate does not yet prove the 
presence of free methyl ester of methyl anthranilic acid. 
Bacillus subtilis^ which is fairly indifferent towards free 
methyl ester of methyl anthranilic acid (compare page 124) 
appears, contrary to the other two micro-organisms, to split 
off a small part of the acetyl group. This conclusion at 
least would seem justified by the slight dirty-blue fluor- 
escence of peptone- water after adding potassium perman- 
ganate. 
Neither blood corpuscles nor hsemachrome undergo any 
change by the action of the acetyl compound of methyl 
ester of methyl anthranilic acid. 
Piperonal. 
Of the six possible dioxybenzaldehydes C^^ H3 (0H)2 • CH O, of 
which three isomers have been obtained by means of chloroform- 
reaction from the three dioxybenzenes (pyrocatechol, resorcinol, and 
hydroquinone), [H2 O -{- C H Clg + C^ (O H)^ = C^ H3 (O • C H O 
-f- 3 HCl] protocatechuic aldehyde is worthy of notice, as it is the 
substance from which piperonal and vanillin are derived — two bodies 
which have recently acquired great importance. 
