— IA — 
20 parts. I treated the structures, cut into as small pieces as possible 
with this fluid for one or several hours (sometimes even for a 
whole day), then teased them in glycerin (50 %) and examined; 
or washed out, and stained with ammo?^ia-carmme or j9/cro-carmme; or 
diluted liæmatoxylin (Delafield's solution) which I, for many purposes, 
can specially recommend, as it gives a very distinct staining, and 
the isolation is not very difflcult afterwards. 
This Hallers maceration-method is very convenient in many 
cases, and is an extremely quick one; the isolation is often possible 
in less than one hour, and in such quickly isolated (or stained and 
isolated) preparations no change of import in the form or structure 
of the elements was perceptible. 
Another, and for some purposes still better, method is with a 
weak solution of Alcoliol. I tried different dilutions from Ranviers 
»Alcohol au tiers^i) (30 7o) down to alcohol of 25 ^/q or 20 % 
and even 17 % (as recommended by Solbrig). I have found the 
weaker solutions especially very good. The structures — freshly 
cut sections or pieces (always as small as possible however) — 
were macerated for one or several days, sometimes even weeks, 
then stained in ammonia- car mine diluted with an equal quantity of 
macerating fluid, for 24 hours, and teased in glycerin of 50 7o- 
Picro-carmin and Delafields liæmatoxylin were also employed as 
staining agents, the latter agent gives, also, here, when employed 
for a short time, good results. 
I usually stain before teasing or isolating, because I think it 
much more practical, and when one is careful not to employ too 
strong solutions, and to dissolve or dilute the staining colours in the 
macerating fluid when possible, it does not at all disturb the facility 
of isolation in any notable degree. 
After having teased the sections or small macerated pieces in 
glycerin, I always protect them with coverglasses on wax-feet, in 
order that I may improve the isolation, if not sufficient, by careful 
knocking on the coverglass with a pencil, or a needle. 
Besides the above mentioned methods I have, of course, also 
How Rawitz arrives at the conclusion he mentions, that Ranvier's 
«Alcohol au tiers«, which consequently is about 30 "/,^, preserves less perfectly 
than the dilution recommended by himself, which contains about 25 alcohol 
or less, and how he, in the latter fluid, can keep the preparations for 6 weeks 
withouth any deformation, whilst in the former fluid fungi grew after a few days, 
I really do not understand. In my opinion it ought to be just the contrary, if any 
difference is to be perceived in that respect. 
