— 8o — 
wooden slides, in the middle of which square apertures are cut to 
suit the glasses. 
This is an exellent method and admits of the employment of 
oil immersions, another advantage is, also, that the sections can be 
examined from both sides, which is often of great importance when 
the sections are thick. For many purposes the above mode of 
mounting on common glass-slides will however do. The preparations 
ought to be kept in darkness when not used. Series of sections 
stained by this silver method were obtained in the following way. 
A piece of the spinal nerve-cord was disected out from a stained 
preparation, and treated quite in the same way as above indi- 
cated for the sections : washed in alcohol, transferred to abs. alcohol, 
then to turpentine. The only difference is that, I let the stream of 
alcohol pass quicker over it. From turpentine it is transferred to a 
solution of paraffin in turpentine which is placed in an incubator heated 
to 56° C. and to which solution paraffin is again added, then into pure 
paraffin and then imbedded. The sections are now made at once, 
then fixed on the slide or coverglass by collodion, and mounted in 
dammar in the above indicated ways. This method may be recom- 
mended also for general purposes, even where no series are required, 
as it is much quicker and more convenient than the other method 
where each section must be treated and mounted separately, though 
it certainly does not afford sections with distinct staining of such 
permanency. 
If convenient, the sections of silver-stained preparations may also 
be stained with colours dissolved in alcohol, e. g. eosin, safranin, 
methylen-blue etc. In this way very nice looking preparations may 
be obtained. 
There are probably a great many ways in which this really 
exellent method of GOLGI may still be improved, and I would re- 
commend it to every histologist of the nervous system, for further 
experiments. 
