DISTRIBUTION OF BACILLUS ENTER1TIDIS SPOROGENES 37 
B. enteritidis. Those 
that 
were non-pathogenic were not 
: accepted as B. en 
teritidis, 
they may have been 
d. en 
tentidis in a non-pathogenic 
form 
of Botkin's 
UULy 1 IV. 
acid bacillus. 
B. ent 
. ? alone. 
B. ent. ? + B.coli. B. coli alone. I] 
. e . , B. ent. 
. ent. tatal. ., 
pathogenic. 
B. ent. non- 
pathogenic. 
Nov. , 1 2 Samples 
6 
0 2 
1 
5 
o 
Dec, 50 Samples ... 
18 
2 0 
12 
5 
3 
Jan., 62 Samples ... 
7 
O 8 
2 
o 
5 
Feb., 55 Samples ... 
7 
I I 
O 
5 
3 
March, 34 Samples ... 
5 
3 0 
3 
5 
o 
Samples sent by the Liverpool Corporation during the first four month 
is of the 
year. I hey were all 
tested 
for B. enteritidis sporogenes 
and 
B. coli in the 
manner 
described above. 
Number of samples. B. enteritiri is alone. 
B. enteritidis + coli. 1 
15. coli alone. 
Shell-fish— Cockles 
17 7 
o 
0 
„ Mussels 
21 4 
2 
0 
„ Periwinkles 
4 4 
O 
o 
„ Oysters 
21 0 
0 
o 
Tinned Meats and Fish 
24 0 
0 
0 
Potted Meats and Sausage 
3 1 
0 
o 
Butter ... 
1 1 0 
0 
0 
Margarine 
3 1 
o 
0 
Cheese 
3 ° 
o 
0 
Sauces 
2 0 
0 
0 
Condensed Milk ... 
3 0 
0 
0 
Jams and Preserves 
6 0 
0 
0 
Tinned Fruits 
4 1 
0 
o 
Flour 
6 3 
o 
0 
Oatmeal 
5 2 
0 
0 
The first 20 samples of grains and the sample of dust were kindly examined 
by Miss Chick for B. coli. The milk samples were examined in the usual course ; 
the method adopted to separate the B coli was to inoculate phenol-agar plates 
(1-1000), which were incubated at 42° C. 
If B. enteritidis had got into the samples from water or dust recently polluted 
with sewage matter one would have expected to find B. coli associated with it ; in the 
case of the grains it is possible that B. coli was there and had died out, leaving the 
more resistant spore-bearing B. enteritidis, so the fact that no B. coli were found 
in the samples of grains and flour examined does not necessarily prove it never was 
present. 
With respect to the milk and shell-fish, however, it is different ; if milk is 
polluted with sewage matter it could only have been recently, so here one would expect 
to find some relation between the presence of B. enteritidis and B. coli. 
In the above samples there does not appear to be any uniform relation between 
the two, for in the larger proportion of samples B. enteritidis occurred alone. 
