BACILLUS COLI AND SALTS OF BILE 
43 
Duration of Life 
As bile salts had an inhibiting effect upon the motility of an actively motile 
B. c. c, while that of B. t. a. was unaffected, it was thought that possibly B. t. a. might 
live longer than B. c. c. in the bile salt medium.* In the first experiment the two 
strains of B. c. c. used died in 9 days, while the B. t. a. lived for some time longer. 
In this and subsequent experiments the incubation temperature was 42 0 C, so that if 
a successful result was obtained the temperature would assist in inhibiting the growth 
of other organisms. The various media used and the duration of life of B. c. c. and 
B. t. a. are given in the appended Table :— 
Organism and Source 
Bile salt 4% 
Pot. ju.ee 
Bile salt 4% 
Pot. Juice 
Bile salt 4% 
Glucose 
Pot. Juice 
Bile salt ^% 
Glucose 
2 -S% 
Pot. Juice 
Bile salt 
Glucose 
t r 0 / 
Pot. Juice 
Bile salt ^ % 
Na N0 3 
1 -5/o 
Water 
Bile salt 5% 
KNO, 
1 r°/ 
1 S/o 
Water 
B. t. a. (Laboratory) 
L 16 
L 12 
D 4 
D S 
D 5 
D 4 
D 5 
„ Human faeces 
L 16 
L 12 
D 2 
D S 
D 5 
D 4 
D 5 
B. c. c. isolated by Eyre from H 2 0... 
D 6 
L 12 
D 7 
D 8 
D 6 
D 7 
D 6 
„ given by Pakes 
D 6 
D 4 
D 4 
D 8 
D 6 
D 5 
D 11 
isolated from H»0 by Pakes 
L 16 
L 12 
L 8 
D 8 
D 4 
D 7 
D 5 
„ ,, human fasces... 
L 16 
D 8 
L 8 
D 8 
D 4 
D 7 
contami- 
nated 
L 16 
L 12 
D 4 
D 8 
D 7 
D 8 
D 10 
„ „ „ horse „ 
L 16 
D 8 
L 8 
D 8 
D 4 
D 7 
D 5 
„ „ ,, cow „ ... 
L 16 
L 12 
D 8 
D 8 
D 4 
D 7 
D 5 
„ „ „ sheep „ 
D 6 
D 6 
L 8 
D 8 
D 5 
D 7 
D 5 
„ „ „ rabbit „ ... 
L 16 
L 12 
D 7 
D 8 
D 6 
L 11 
D 5 
„ dog „ ... 
D 6 
D 6 
D 5 
D 6 
D 4 
D 5 
„ „ „ mouse „ ... 
L 12 
D + 
D 8 
D 8 
L 11 
D .3 
„ „ „ Ice Cream by 
Pakes 
D 6 
L 12 
L 8 
D 8 
D 11 
D 5 
„ „ „ blood of case 
of Infective Endocarditis 
D 7 
D 4 
D 7 
Typhoid stools 
D 5 
L ,5 
Gaertner, given by Durham... 
L 16 
L 12 
L 8 
D 8 
D 12 
L 11 
L 15 
L=living. D = dead. The numbers indicate the days after inoculation. 
In order to ascertain whether a culture was living the bile salt tube was well 
shaken, and a loopful transferred to ordinary broth, and this broth tube incubated at 
* A percentage of 4 was used because the effect upon the motility of B. c. c. was distinct. 
