44 
THOMPSON YATES LABORATORIES REPORT 
37° C. If the broth cultures did not show any growth the transplantations from the 
bile salt medium were still continued for several days, so as to make quite certain that 
there were no viable organisms present. 
The results are negative as regards both differentiating B. t. a. from B. c. c, 
and isolating B. t. a. from mixtures. But they show that 2.5 p.c. of glucose has a 
distinctly inhibiting effect upon the organisms used. 
This action of glucose is confirmed by the following experiment. Faeces from 
1. A case of Bright's Disease, 
2. A case of Mitral Stenosis, 
3. A case of Cirrhosis with ascites, 
were inoculated into tubes containing 
A. Bile salt 5 p.c. KNO3 1.5 p.c. Water, 
B. Bile salt 5 p.c. NaN0 3 1.5 p.c. Water, 
C. Bile salt 5 p.c. Glucose 2.5 p.c. Potato Juice, 
and incubated at 42" C. 
1 A. Was living on 14th day. 
1 B. Was living on 14th day. 
1 C. Was dead on 5th day. 
2 A. Was living on 14th day. 
2 B. Was living on 14th day. 
2 C. Was dead on 5th day. 
3 A. Was living on 14th day. 
3 B. Was living on 14th day. 
3 C. Was dead on 6th day. • 
The latest culture which shewed any growth was plated in ordinary nutrient 
gelatine. 
The organisms isolated were — From 
1 A. An organism which looked like a streptococcus, and which gave 
Broth — General turbidity, 
Litmus Milk — Acid, but no clotting, 
Potato — Raised whitish growth, 
Gel. S. tab — Very slight growth on surface, but good growth all down 
the needle track. 
1 B. B. c. c. 
1 C. {a) B. c. c. 
(/?) A bacillus which produced slight fluorescence in gelatine, caused no 
change in milk, and gave a moist yellowish growth, with 
discolouration of potato. 
2 A. Organism similar to 1 A. 
2 B. B. c. c. 
2 C. (a) B. c. c. 
{b) Organism similar to 1 C. 
3 A. Liquefying motile bacillus, which peptonizes milk, turning it brownish 
yellow, gives slight indol, but no gas ; produces general turbidity 
in broth, and a moist brownish-yellow growth, with discolouration 
of potato. 
3 B. Same as 3 A. 
3 C. B. c. c. 
