BACILLUS COLI AND SALTS OF BILE 45 
From these three cases, then, there were isolated four organisms which would 
grow in bile salt 5 p.c. media at 42 0 C, and the most frequent was the B. c. c. 
It was thought that an increased percentage of bile salt might inhibit all but 
B. c. c. [That B. c. c. would grow in 7 p.c. B. s. was proved by the experiment 
given in the following Table : — 
Medium — Bile salt 7 p.c, KN0 3 , NaN0 3) AmCl., each 0.5 p.c. Made up with 
water. Temperature — 42 0 C. 
B. t. a. faeces . . . Dead 5th day. 
B. c. c. . . . Living 10th day. 
„ Infec. Endocarditis 
„ Ice Cream . 
,, Cow 
„ Horse 
,, Sheep 
„ Dog 
Gaertner . . . . „ „ ] 
So faeces from (1) a case of phosphorous poisoning, (2) a case of (?) influenza, 
were inoculated into tubes containing the above medium, and incubated at 42 0 C. for 
5 days, and then plates were made from these tubes. 
From case 1 were obtained (a) a liquefying motile bacillus, similar to the one 
which had been isolated from previous case ; (b) a non-typical B. c. c. From case 2, 
only B. c. c. was isolated. 
Fasces from the Rabbit, Sheep, and Cow were inoculated into the same medium, 
and incubated at 42 0 C, for 48 hours, and then plated in ordinary gelatine. 
The organisms isolated were : — From Rabbit, only B. c. c. group ; Sheep, 
B. c. c, and the same liquefying motile bacillus as before ; Cow, B. c. c. group only. 
The experiment was repeated with two specimens of Rabbit fasces, and the 
organisms isolated were : — (a) B. c. c. ; (b) a motile liquefying bacillus having the 
same characteristics as before. 
A repetition with faeces of the Cow and Sheep resulted in the isolation of 
B. c. c. only. 
As glucose seemed to have an unfavourable effect upon the duration of life of 
B. c. c. and B. t. a. a liquid medium was made, consisting of potato juice containing 
varying percentages of glucose, and B. c. c. and B. t. a. were grown in this medium at 
37° C. The amounts of the sugar used were 1, 2, 3, and 4 per cent. 
Bacillus Typhi Abdominalis 
Motility 
The lowest percentage causes a decrease in the motility as compared with 
ordinary broth, and as the percentage increases the amount of movement 
decreases. 
